数字农科院2.0

Cr3a, a candidate gene conferring fruit cracking resistance, was fine-mapped in an introgression line of Solanum lycopersicum L.

文献类型： 外文期刊

作者： Yifan Chen;Wenzheng Gao;Yu Zhu;Shuliang Qiu;Zhuoyao Qiu;Chenchen Dong;Ziteng Liu;Yongchen Du;Junming Li;Zejun Huang;Xin Li;Lei Liu;Liwang Liu;Xiaoxuan Wang

作者机构：

关键词： Cr3a;fruit cracking;gene editing;map-based cloning;metabolome;tomato;transcriptome

期刊名称： Plant Journal

ISSN： 0960-7412

年卷期： 2025 年 122 卷 2 期

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： In the cultivation and production of tomato (Solanum lycopersicum L.), fruit cracking is a prevalent and detrimental issue that significantly impacts the esthetic quality and commercial value of the fruit. The complexity of the trait has resulted in a slow advancement in research aimed at identifying genes that influence tomato fruit cracking and the underlying regulatory mechanisms. In this study, a sub-introgression population for tomato crack-resistant fruit has been constructed from the cross between S. lycopersicum 1052 and Solanum pennellii LA0716, followed by 11 generations of selfing. Utilizing specifically designed InDel markers, the tomato crack-resistant gene, Cr3a, was fine-mapped, cloned, and its functionality was confirmed through transgenic and gene-knockout approaches. The precise localization of Cr3a was delineated to a 30 kb genomic region on chromosome 3, corresponding to the gene Sopen03g034650 in S. pennellii and Solyc03g115660.3 in the Heinz1706 variety. An integrated transcriptomic and metabolomic analysis of fruits with and without the Cr3a gene was finally conducted to elucidate the intricate regulatory mechanisms associated with Cr3a. The findings revealed a molecular regulatory network for tomato fruit crack resistance, characterized by 7 key metabolites, 13 pivotal genes, and 4 critical pathways: the phenylpropanoid biosynthesis pathway, the phenylalanine, tyrosine, and tryptophan biosynthesis pathway, the linolenic acid metabolism pathway, and the cysteine and methionine metabolism pathway. In summary, this research provides novel insights into the molecular underpinnings of tomato fruit crack resistance and holds substantial promise for accelerating the molecular breeding of tomatoes with enhanced fruit crack resistance.

分类号：

  • 相关文献

[1]Candidate Gene Identification and Transcriptome Analysis of Tomato male sterile-30 and Functional Marker Development for ms-30 and Its Alleles, ms-33, 7B-1, and stamenless-2. Kai Wei,Xin Li,Xue Cao,Shanshan Li,Li Zhang,Feifei Lu,Chang Liu,Yanmei Guo,Lei Liu,Can Zhu,Yongchen Du,Junming Li,Wencai Yang,Zejun Huang,Xiaoxuan Wang. 2024

[2]Fine Mapping and Candidate Gene Validation of Tomato Gene Carpelloid Stamen and Parthenocarpy (CSP). Shanshan Li,Kai Wei,Li Zhang,Yu Ning,Feifei Lu,Xiaoxuan Wang,Yanmei Guo,Lei Liu,Xin Li,Can Zhu,Yongchen Du,Junming Li,Zejun Huang. 2024

[3]Resistance of the CRISPR-Cas13a Gene-Editing System to Potato Spindle Tuber Viroid Infection in Tomato and Nicotiana benthamiana. Ying Wei Khoo,Qingsong Wang,Shangwu Liu,Binhui Zhan,Tengfei Xu,Wenxia Lv,Guangjing Liu,Shifang Li,Zhixiang Zhang. 2024

[4]Comparative transcriptome profiling of Eimeria tenella in various developmental stages and functional analysis of an ApiAP2 transcription factor exclusively expressed during sporogony. Linlin Chen,Xinming Tang,Pei Sun,Dandan Hu,Yuanyuan Zhang,Chaoyue Wang,Junmin Chen,Jie Liu,Yang Gao,Zhenkai Hao,Ning Zhang,Wenxuan Chen,Fujie Xie,Xun Suo,Xianyong Liu. 2023

[5]Transcriptomic Change in the Effects of Dichloroquinolinic Acid on the Development and Growth of Nicotiana tabacum. Bingjie Li,Anming Ding,Weifeng Wang,Mengmeng Cui,Yuhe Sun,Jing Lv,Changbo Dai. 2024

[6]Multi-omics integration to explore the molecular insight into the volatile organic compounds in watermelon. Chengsheng Gong,Nan He,Hongju Zhu,Muhammad Anees,Xuqiang Lu,Wenge Liu. 2023

[7]Defensive Resistance of Cowpea Vigna unguiculata Control Megalurothrips usitatus Mediated by Jasmonic Acid or Insect Damage. Tao Li,Mingyue Feng,Yuanming Chi,Xing Shi,Zilin Sun,Zhen Wu,Aomei Li,Wangpeng Shi. 2023

[8]Transcriptomic and metabolomic analyses reveal that exogenous strigolactones alleviate the response of melon root to cadmium stress. Chen X.,Shi X.,Ai Q.,Han J.,Wang H.,Fu Q.. 2022

[9]Transcriptome Co-expression Network and Metabolome Analysis Identifies Key Genes and Regulators of Proanthocyanidins Biosynthesis in Brown Cotton. Zhenzhen Wang,Xiaomeng Zhang,Shoupu He,Abdul Rehman,Yinhua Jia,Hongge Li,Zhaoe Pan,Xiaoli Geng,Qiong Gao,Liru Wang,Zhen Peng,Xiongming Du. 2022

[10]Transcriptomic and Metabolomic Analysis of Wheat Kernels in Response to the Feeding of Orange Wheat Blossom Midges (Sitodiplosis mosellana) in the Field. Qian Wang,Xiaobei Liu,Huan Liu,Yu Fu,Yumeng Cheng,Lijiao Zhang,Wangpeng Shi,Yong Zhang,Julian Chen. 2022

[11]Integrated Transcriptome and Metabolome Dissecting Interaction between Vitis vinifera L. and Grapevine Fabavirus. Baodong Zhang,Mengyan Zhang,Xiaojun Jia,Guojun Hu,Fang Ren,Xudong Fan,Yafeng Dong. 2023

[12]Integrated Metabolome and Transcriptome Analysis Unveils the Underlying Molecular Response of Panax ginseng Plants to the Phytophthora cactorum Infection. Hong Kan,Shuai Qu,Kai Dong,Shihan Wang,Chen Xu,Yingping Wang,Shuang Hua. 2023

[13]Integrated metabolomics and transcriptomics insights on flavonoid biosynthesis of a medicinal functional forage, Agriophyllum squarrosum (L.), based on a common garden trial covering six ecotypes. Tingzhou Fang,Shanshan Zhou,Chaoju Qian,Xia Yan,Xiaoyue Yin,Xingke Fan,Pengshu Zhao,Yuqiu Liao,Liang Shi,Yuxiao Chang,Xiao Fei Ma. 2022

[14]Multi-omics analyses of 398 foxtail millet accessions reveal genomic regions associated with domestication, metabolite traits, and anti-inflammatory effects. Xukai Li,Jianhua Gao,Jingyi Song,Kai Guo,Siyu Hou,Xingchun Wang,Qiang He,Yanyan Zhang,Yakun Zhang,Yulu Yang,Jiaoyan Tang,Hailang Wang,Staffan Persson,Mingquan Huang,Lishuai Xu,Linlin Zhong,Dongqin Li,Yongming Liu,Hua Wu,Xianmin Diao,Pe. 2022

[15]Integrative analysis of metabolome and genome-wide transcriptome reveal the flavor changes in apple (Malus pumila Mill) after the novel acaricide cyflumetofen application. Minmin Li,Lin Li,Zhiqiang Kong,Noel Gregoire,Rui Quan,Zisheng Luo,Xingyu Lin,Jesus Simal-Gandara,Bei Fan,Fengzhong Wang. 2023

[16]Comparison of transcriptome and metabolome analysis revealed differences in cold resistant metabolic pathways in different apple cultivars under low temperature stress. Xu, Gongxun,Li, Lijie,Zhou, Jia,Lyu, Deguo,Zhao, Deying,Qin, Sijun. 2023

[17]Combining quantitative trait locus mapping with multiomics profiling reveals genetic control of corn leaf aphid (Rhopalosiphum maidis) resistance in maize. Wang, Tengyue,Wang, Kaiji,Wang, Chuanhong,Zhao, Yibing,Tao, Zhen,Li, Junyao,Wang, Lei,Shi, Jian,Huang, Shijie,Xie, Chuanxiao,Li, Peijin. 2023

[18]Editorial: Omics data-based identification of plant specialized metabolic genes. Peipei Wang,Pengxiang Fan,Yan Bao,Wei Li,Li Wang. 2023

[19]Metabolome and Transcriptome Analyses Reveal the Differences in the Molecular Mechanisms of Oat Leaves Responding to Salt and Alkali Stress Conditions. Bai, Jianhui,Lu, Peina,Li, Feng,Li, Lijun,Yin, Qiang. 2023

[20]Metabolome and Transcriptome Integration Reveals Insights Into Flavor Formation of ‘Crimson’ Watermelon Flesh During Fruit Development. Chengsheng Gong,Weinan Diao,Hongju Zhu,Muhammad Jawad Umer,Shengjie Zhao,Nan He,Xuqiang Lu,Pingli Yuan,Muhammad Anees,Dongdong Yang,M. O. Kaseb,Wenge Liu. 2021

作者其他论文 更多>>