数字农科院2.0

Characterization and expression analysis of Gossypium NLP family reveals the roles of GhNLP2.1 contributing to nitrogen use efficiency in upland cotton

文献类型： 外文期刊

作者： Yunqi Gu;Xiaotong Li;Asif Iqbal;Xiangru Wang;Tong Luo;Qianqian Wang;Yuanchun Pu;Huiping Gui;Meizhen Song;Qiang Dong

作者机构：

关键词： Bioinformatics analysis;Gene expression;Gossypium hirsutum;NIN-like protein;Nitrogen Use Efficiency;Virus-induced gene silencing

期刊名称： Industrial Crops and Products

ISSN： 0926-6690

年卷期： 2025 年 235 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： NIN-like proteins (NLPs) are evolutionarily conserved transcription factors that play a key role in regulating plant physiological responses to changes in external nitrogen levels. Although NLP genes have been extensively studied in various plant species, a comprehensive analysis of the NLP gene family in cotton (Gossypium spp.) remains lacking. In this study, 65 NLP genes were identified across four cotton species (G. raimondii, G. barbadense, G. arboreum, and G. hirsutum) and were classified into three groups. Analyses of protein characteristics, phylogeny, gene structure, conserved motifs, and gene expression were conducted. Gene family expansion of GhNLPs was primarily driven by fragment duplication. RNA-seq and qRT-PCR analyses showed that GhNLP2.1 was significantly upregulated by nitrogen treatments in cotton roots. Its expression level increased in response to higher nitrogen concentrations. Overexpression of GhNLP2.1 in Arabidopsis resulted in improved growth and enhanced activities of nitrogen metabolism-related enzymes under low nitrogen conditions compared to sufficient nitrogen conditions. Silencing of GhNLP2.1 through virus-induced gene silencing significantly impaired nitrogen accumulation and utilization in cotton. This study enhances our understanding of GhNLP genes, highlights their role in regulating nitrogen use efficiency in upland cotton, and provides a foundation for future research on GhNLP2.1 function.

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