数字农科院2.0

Two Magnaporthe appressoria-specific (MAS) proteins, MoMas3 and MoMas5, are required for suppressing host innate immunity and promoting biotrophic growth in rice cells

文献类型： 外文期刊

作者： Gong, Ziwen;Ning, Na;Li, Zhiqiang;Xie, Xin;Wilson, Richard A.;Liu, Wende

作者机构：

关键词： apoplastic effector;Magnaporthe appressoria-specific (MAS) proteins;Magnaporthe oryzae;plant immunity;virulence factor

期刊名称： MOLECULAR PLANT PATHOLOGY

ISSN： 1464-6722

年卷期： 2022 年

页码：

收录情况： SCIE（2022版）

摘要： In the devastating rice blast fungus Magnaporthe oryzae, six Magnaporthe appressoria-specific (MAS) proteins are encoded by MoGAS1, MoGAS2 and MoMAS3-MoMAS6. MoGAS1 and MoGAS2 were previously characterized as M. oryzae virulence factors; however, the roles of the other four genes are unknown. Here, we found that, although the loss of any MAS gene did not affect appressorial formation or vegetative growth, increment Momas3 and increment Momas5 mutant strains (but not the others) were reduced in virulence on susceptible CO-39 rice seedlings. Focusing on increment Momas3 and increment Momas5 mutant strains, we found that they could penetrate host leaf surfaces and fill the first infected rice cell but did not spread readily to neighbouring cells, suggesting they were impaired for biotrophic growth. Live-cell imaging of fluorescently labelled MoMas3 and MoMas5 proteins showed that during biotrophy, MoMas3 localized to the apoplastic compartment formed between fungal invasive hyphae and the plant-derived extra-invasive hyphal membrane while MoMas5 localized to the appressoria and the penetration peg. The loss of either MoMAS3 or MoMAS5 resulted in the accumulation of reactive oxygen species (ROS) in infected rice cells, resulting in the triggering of plant defences that inhibited mutant growth in planta. increment Momas3 and increment Momas5 biotrophic growth could be remediated by inhibiting host NADPH oxidases and suppressing ROS accumulation. Thus, MoMas3 and MoMas5 are novel virulence factors involved in suppressing host plant innate immunity to promote biotrophic growth.

分类号：

  • 相关文献

[1]Plant innate immunity in rice: a defense against pathogen infection. Wang, Guo-Liang,Wang, Guo-Liang. 2016

[2]A VQ-motif-containing protein fine-tunes rice immunity and growth by a hierarchical regulatory mechanism. Hao Z.,Tian J.,Fang H.,Fang L.,Xu X.,He F.,Li S.,Xie W.,Du Q.,You X.,Wang D.,Chen Q.,Wang R.,Zuo S.,Yuan M.,Wang G.-L.,Xia L.,Ning Y.. 2022

[3]Protein elicitor AMEP412 suppressed rice blast caused by Magnaporthe oryzae through triggering plant immunity. Yazhi Qin,Meijun Guo,Junyan Yang,Jiayi Zheng,Quan Wang,Quan Liu. 2025

[4]麦瘟病研究进展与展望. 何心尧,郝元峰,周益林,何中虎. 2017

[5]Purification, characterization. and gene cloning of an alkaline serine protease from a highly virulent strain of the nematode-endoparasitic fungus Hirsutella rhossiliensis. Liu, Xingzhong,Wang, Bin,Liu, Xiaoying,Wu, Wenping,Li, Shidong. 2009

[6]Complete Genome Sequence of Aeromonas veronii Strain B565. Liu, Yuchun,Zhang, Yuting,Zhou, Zhigang,Li, Yanxia,Zhou, Zhemin,Huang, Huoqing,Ren, Yan,Li, Guannan,Wang, Lei,Zhou, Zhemin,Ren, Yan,Wang, Lei,Ren, Yan,Wang, Lei.

[7]Genome-Wide Analysis Of The First S.equenced Mycoplasma Capricolum Subsp C apripneumoniae Strain M1601. Chen, SL,Hao, HF,Zhao, P,Thiaucourt, F,He, Y,Gao, PC,Guo, H,Ji, WH,Wang, ZH,Lu, ZX,Chu, YF,Liu, YS. 2017

[8]Long Non-Coding RNAs profiling in pathogenesis of Verticillium dahliae: New insights in the host-pathogen interaction. Nana Liu,Ping Wang,Xiancai Li,Yakun Pei,Yun Sun,Xiaowen Ma,Xiaoyang Ge,Yutao Zhu,Fuguang Li,Yuxia Hou. 2022

[9]Extracellular DNase MAP3916c attacks the neutrophil extracellular traps and is needed for Mycobacterium avium subsp. paratuberculosis virulence. Xinxin Zang,Guanghui Dang,Zhuming Cai,Mingzhu Shao,Yangyang Tang,Jun Cao,Ziyin Cui,Siguo Liu. 2022

[10]Thioredoxin VdTrx1, an unconventional secreted protein, is a virulence factor in Verticillium dahliae. Li Tian,Jing Zhuang,Jun Jiao Li,He Zhu,Steven J. Klosterman,Xiao Feng Dai,Jie Yin Chen,Krishna V. Subbarao,Dan Dan Zhang. 2023

[11]Identification Of The Potential Virulence F.actors And Rna Silencing S uppressors Of Mulberry Mosaic Dwarf-Associated Geminivirus. Yang, XL,Ren, YX,Sun, SS,Wang, DX,Zhang, FF,Li, DW,Li, SF,Zhou, XP. 2018

[12]Inhibition of Haemophilus parasuis by berberine and proteomic studies of its mechanism of action. Yue Jia,Changqi Hao,Qin Yang,Wanjiang Zhang,Gang Li,Siguo Liu,Xin Hua. 2021

[13]MGF360-9L Is a Major Virulence Factor Associated with the African Swine Fever Virus by Antagonizing the JAK/STAT Signaling Pathway. Keshan Zhang,Bo Yang,Chaochao Shen,Ting Zhang,Yu Hao,Dajun Zhang,Huanan Liu,Xijuan Shi,Guoli Li,Jinke Yang,Dan Li,Zixiang Zhu,Hong Tian,Fan Yang,Yi Ru,Wei Jun Cao,Jianhong Guo,Jijun He,Haixue Zheng,Xiangtao Liu. 2022

[14]Reprogramming of phytopathogen transcriptome by a non-bactericidal pesticide residue alleviates its virulence in rice. Haruna Matsumoto,Yuan Qian,Xiaoyan Fan,Sunlu Chen,Yanxia Nie,Kun Qiao,Dandan Xiang,Xinzhong Zhang,Meng Li,Bo Guo,Peilin Shen,Qiangwei Wang,Yunlong Yu,Tomislav Cernava,Mengcen Wang. 2022

[15]Pmorf0222, a Virulence Factor in Pasteurella multocida, Activates Nuclear Factor Kappa B and Mitogen-Activated Protein Kinase via Toll-Like Receptor 1/2. Xu, Tenglin,Zheng, Yating,Liu, Benjin,Kou, Mingxing,Jiang, Qian,Liu, Jiasen,Kang, Hongtao,Yang, Mingfa,Guo, Dongchun,Qu, Liandong. 2023

[16]Deletion of a 7-amino-acid region in the porcine epidemic diarrhea virus envelope protein induces higher type I and III interferon responses and results in attenuation in vivo. Zhiwei Li,Zhiqian Ma,Weiguo Han,Chuanzhe Chang,Yang Li,Xuyang Guo,Zifang Zheng,Yingtong Feng,Lele Xu,Haixue Zheng,Xinglong Wang,Shuqi Xiao. 2023

[17]PMCNA_RS00975 activates NF-κB and ERK1/2 through TLR2 and contributes to the virulence of Pasteurella multocida. Xu, Tenglin,Kou, Mingxing,Cao, Peili,Liu, Benjin,Zheng, Yating,Jiang, Qian,Liu, Jiasen,Kang, Hongtao,Yang, Mingfa,Guo, Dongchun,Qu, Liandong. 2024

[18]A Sec-dependent effector, CLIBASIA_04425, contributes to virulence in 'Candidatus Liberibater asiaticus'. Zhang, Shushe,Wang, Xuefeng,He, Jun,Zhang, Song,Zhao, Tingchang,Fu, Shimin,Zhou, Changyong. 2023

[19]Genomic insight into the diversity of Glaesserella parasuis isolates from 19 countries. Gong, Xiaowei,Cui, Qingpo,Zhang, Wanjiang,Shi, Yuqian,Zhang, Peng,Zhang, Chaoyang,Hu, Gongzheng,Sahin, Orhan,Wang, Lu,Shen, Zhangqi,Fu, Mengjiao. 2024

[20]Water-saving irrigation suppresses on soil-crop pathogenicity: Insight on the co-host of virulence factors and antibiotic resistance genes. Lizhi Zhao,Yuhui Li,Qifan Yang,Ruolan Li,Houyu Li,Yan Xu. 2025

作者其他论文 更多>>