数字农科院2.0

A Targeted RNAi-of-RNAi Strategy to Overcome Nuclease-Mediated RNAi Suppression and Enhance RNAi Efficacy in Lepidoptera Pest, Cydia Pomonella

文献类型： 外文期刊

作者： Li, Xiaojiang;Fu, Lianhai;Lu, Yin;Wu, Qiang;Qiao, Xi;Wan, Fanghao;Liu, Bo;Qian, Wanqiang;Li, Zaiyuan

作者机构：

关键词： （1-1-2）Cydia pomonella;RNA interference;REase gene;dsRNA degradation;RNAi-of-RNAistrategy

期刊名称： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ISSN： 0021-8561

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： The codling moth has developed resistance to chemical insecticides and biological agents, necessitating alternative control strategies. RNA interference (RNAi) offers considerable potential. However, variable efficacy across target genes remains a major barrier in lepidopterans. In this study, we identified three REase genes in the codling moth, each containing a conserved PIN domain. CmREase1 and CmREase2 were strongly induced by exogenous dsRNA and showed high expression in midgut and hemolymph. RNAi-mediated silencing of these genes reduced dsRNA degradation in hemolymph and significantly enhanced RNAi efficiency, confirming their key roles in dsRNA degradation and suppression of RNAi. In contrast, CmREase4 was dsRNA-responsive but had no detectable effect on RNAi efficiency. Moreover, transcriptional profiling of Dcr-2 and AGO2 revealed that pretreatment with nonspecific dsEGFP followed by target-specific dsRNA markedly improved RNAi efficacy, comparable to REase knockdown. Collectively, these results demonstrate that REases are critical modulators of RNAi efficiency in codling moth and provide a framework for RNAi-based biopesticide development, offering new strategies to overcome variability in RNAi responsiveness among lepidopteran pests.

分类号：

  • 相关文献

[1]Molecular and Functional Characterization of Three General Odorant-Binding Protein 2 Genes in Cydia pomonella (Lepidoptera: Tortricidae). Yanan Zhou,Cong Huang,Guanjun Fu,Rui Tang,Nianwan Yang,Wanxue Liu,Wanqiang Qian,Fanghao Wan. 2024

[2]Knockdown of a putative alanine aminotransferase gene affects amino acid content and flight capacity in the Colorado potato beetle Leptinotarsa decemlineata. Wan, Pin-Jun,Fu, Kai-Yun,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao,Wan, Pin-Jun.

[3]Effective inhibition of Japanese encephalitis virus replication by small interfering RNAs targeting the NS5 gene. Qi, Wen-Bao,Hua, Rong-Hong,Yan, Li-Ping,Tong, Guang-Zhi,Wu, Dong-Lai,Qi, Wen-Bao,Tong, Guang-Zhi,Zhang, Gui-Hong,Ren, Tao,Liao, Ming. 2008

[4]The effect of silencing arginine kinase by RNAi on the larval development of Helicoverpa armigera. Su, X. -F.,Lu, G. -Q.,Cheng, H. -M.,Liu, C. -X.,Liang, G. -M..

[5]Silencing in Apolygus lucorum of the olfactory coreceptor Orco gene by RNA interference induces EAG response declining to two putative semiochemicals. Zhu, Xiao-Qiang,Gu, Shao-Hua,Cui, Huan-huan,Guo, Yu-Yuan,Zhang, Yong-Jun,Zhou, Jing-Jiang. 2014

[6]In vitro inhibition of transmissible gastroenteritis coronavirus replication in swine testicular cells by short hairpin RNAs targeting the ORF 7 gene. He, Lei,Zhang, Yan-ming,Dong, Ling-juan,Cheng, Min,Wang, Jing,Tang, Qing-hai,Wang, Gang,Tang, Qing-hai. 2012

[7]Knockdown of CDK2AP1 by RNA interference inhibits cell growth and tumorigenesis of human glioma. Xu, Yonggang,Wang, Zhi,Wang, Jie,Fu, Songbin. 2014

[8]Reduction of infectious bursal disease virus replication by shRNAs targeting the VP1 and VP2 genes driven by chicken U6 promoter. Wei Ouyang,Ma, Jin-rong,Jiang, Jie-yuan,Wang, Yong-shan,Wang, Yong-qiang,Qin, Li-ting,Wang, Xiao-mei,Fan, Hong-jie. 2013

[9]In vitro and in vivo evaluation of small interference RNA-mediated gynaecophoral canal protein silencing in Schistosoma japonicum. Cheng, Guofeng,Fu, Zhiqiang,Lin, Jiaojiao,Cai, Youmin,Shi, Yi,Zhou, Yuancong,Jin, Youxin. 2009

[10]RNAi in the striped stem borer, Chilo suppressalis, establishes a functional role for aminopeptidase N in Cry1Ab intoxication. Du, L. X.,Liu, C. X.,Han, L. Z.,Peng, Y. F.,Gong, L..

[11]Silencing of molt-regulating transcription factor gene, CiHR3, affects growth and development of sugarcane stem borer, Chilo infuscatellus. Zhang, Shu-zhen,Wu, Su-ran,Yu, Nai-tong,Wang, Jian-hua,Liu, Zhi-xin,Kulye, Mahesh,Zeng, Hong-mei. 2012

[12]Role of non-structural protein 2 in the regulation of the replication of the porcine reproductive and respiratory syndrome virus in MARC-145 cells: Effect of gene silencing and over expression. Wang, Feng-Xue,Wen, Yong-Jun,Yang, Bo-Chao,Liu, Zhun,Shi, Xin Chuan,Leng, Xue,Song, Ni,Wu, Hua,Chen, Li-Zhi,Cheng, Shi-Peng.

[13]Effects of injecting cadherin gene dsRNA on growth and development in diamondback moth Plutella xylostella (Lep.: Plutellidae). Yang, Z. -X.,Wu, Q. -J.,Wang, S. -L.,Xu, B. -Y.,Chang, X. -L.,Zhu, G. -R.,Zhang, Y. -J.,Yang, Z. -X.,Wen, L. -Z..

[14]Chrysopa septempunctata (Neuroptera: Chrysopidae) Vitellogenin Functions Through Effects on Egg Production and Hatching. Liu, C.,Mao, J.,Zeng, F.,Liu, C..

[15]In vitro Study of Nucleostemin as a Potential Therapeutic Target in Human Breast Carcinoma SKBR-3 Cells. Guo, Yu,Liao, Ya-Ping,Zhang, Ding,Xu, Li-Sha,Li, Na,Liu, Chang-Qing,Guan, Wei-Jun,Liu, Chang-Qing. 2014

[16]RNAi-mediated plant protection against aphids. Yu, Xiu-Dao,Sun, Yong-Wei,Ma, You-Zhi,Xia, Lan-Qin,Yu, Xiu-Dao,Liu, Zong-Cai,Huang, Si-Liang,Chen, Zhi-Qin,Duan, Peng-Fei.

[17]The effect of myostatin silencing by lentiviral-mediated RNA interference on goat fetal fibroblasts. Lu, Jian,Wei, Caihong,Zhang, Xiaoning,Xu, Lingyang,Zhang, Shifang,Liu, Jiasen,Cao, Jiaxue,Zhao, Fuping,Zhang, Li,Du, Lixin,Lu, Jian,Lu, Jian,Li, Bichun,Liu, Jiasen,Cao, Jiaxue.

[18]Isolation and characterization of two novel serpins from the tick Rhipicephalus haemaphysaloides. Yu, Yingfang,Cao, Jie,Zhou, Yongzhi,Zhang, Honshuang,Zhou, Jinlin. 2013

[19]Selection of an effective small interference RNA to silence myostatin gene expression in sheep fibroblast cells. Lu, Jian,Ding, Jiatong,Li, Bichun,Lu, Jian,Xu, Lingyang,Lu, Guobin,Zhao, Fuping,Wei, Caihong,Zhang, Li,Du, Lixin,Sun, Dan.

[20]Inhibition of avian leukosis virus subgroup J replication by miRNA targeted against env. Wang, Wei,Zhang, Zai-Ping,Tian, Jin,Xiao, Zhi-Guang,Meng, Qing-Wen.

作者其他论文 更多>>