数字农科院2.0

Biocontrol potential and mechanism of a seed-endophytic Bacillus velezensis NEAU-HLD-9 against gray mold in green beans

文献类型： 外文期刊

作者： Zhang, Kuan;Yu, Xiaoyan;Yang, Xinru;Yang, Zhiqi;Zhang, Yan;Liu, Jiayi;Wang, Xiangjing;Zhao, Junwei;Xiang, Wensheng

作者机构：

关键词： Green bean;Gray mold;Bacillus velezensis;Biological control;Antifungal mechanism

期刊名称： FOOD MICROBIOLOGY

ISSN： 0740-0020

年卷期： 2025 年 136 卷

页码：

收录情况： SCIE（2025版）

摘要： Gray mold caused by Botrytis cinerea has a severe impact on postharvest green beans, leading to quality degradation and substantial economic losses. The application of plant endophytic microorganisms has proven to be a reliable and safe strategy for controlling fungal diseases. In the present study, an endophytic bacterium, Bacillus velezensis NEAU-HLD-9, was isolated from healthy bean seeds and exhibited strong inhibitory activity against Botrytis cinerea. This strain could form biofilms, enhance the activity of defense enzymes, and boost the content of active secondary metabolites in green beans. The crude lipopeptide extracts from NEAU-HLD-9 remarkably suppressed the mycelial growth and spore germination of B. cinerea. It is worth noting that the crude lipopeptide extracts also effectively controlled gray mold in green beans by reducing both disease incidence and lesion diameter. PCR and LC-MS analyses suggested that the crude lipopeptide extracts comprised two antifungal compounds. Whole genome sequencing revealed that the strain contained 15 gene clusters responsible for secondary metabolite synthesis, including those for antifungal compounds. In summary, both Bacillus velezensis NEAU-HLD-9 and its crude lipopeptide extracts strongly inhibited Botrytis cinerea in vitro and in vivo, suggesting a promising approach for the management of gray mold in green beans.

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