数字农科院2.0

Cloning and sequence analysis of sucrose phosphate synthase gene from varieties of Pennisetum species

文献类型： 外文期刊

作者： Li, H. C.;Lu, H. B.;Yang, F. Y.;Liu, S. J.;Zhang, Y. W.;Li, H. C.;Lu, H. B.;Yang, F. Y.;Liu, S. J.;Zhang, Y. W.;Bai, C. J.

作者机构：

关键词： Pennisetum Rich.;Sucrose phosphate synthase;Gene clone;Sequence analysis

期刊名称： GENETICS AND MOLECULAR RESEARCH

ISSN： 1676-5680

年卷期： 2015 年 14 卷 1 期

页码：

收录情况： SCI

摘要： Sucrose phosphate synthase (SPS) is an enzyme used by higher plants for sucrose synthesis. In this study, three primer sets were designed on the basis of known SPS sequences from maize (GenBank: NM_001112224.1) and sugarcane (GenBank: JN584485.1), and five novel SPS genes were identified by RT-PCR from the genomes of Pennisetum spp (the hybrid P. americanum x P. purpureum, P. purpureum Schum., P. purpureum Schum. cv. Red, P. purpureum Schum. cv. Taiwan, and P. purpureum Schum. cv. Mott). The cloned sequences showed 99.9% identity and 80-88% similarity to the SPS sequences of other plants. The SPS gene of hybrid Pennisetum had one nucleotide and four amino acid polymorphisms compared to the other four germplasms, and cluster analysis was performed to assess genetic diversity in this species. Additional characterization of the SPS gene product can potentially allow Pennisetum to be exploited as a biofuel source.

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