数字农科院2.0

Boosting the cellulolytic oxidative and hydrolytic enzyme systems in Myceliophthora thermophila for the efficient enzymatic saccharification of lignocellulosic biomass

文献类型： 外文期刊

作者： Bai, Rui;Yang, Kun;Wang, Yaru;Wang, Yuan;Wang, Xiaolu;Tu, Tao;Zhang, Jie;Su, Xiaoyun;Huang, Huoqing;Yao, Bin;Luo, Huiying;Qin, Xing

作者机构：

关键词： Lytic polysaccharide monooxygenase;beta-glucosidase;Myceliophthora thermophila;Enzymatic saccharification;Lignocellulosic biomass

期刊名称： BIORESOURCES AND BIOPROCESSING

ISSN：

年卷期： 2025 年 12 卷 1 期

页码：

收录情况： SCIE（2025版） ; ; EI（2025版） ; ; CSCD（2025-2026年度）

摘要： The thermophilic fungus Myceliophthora thermophila serves as a vital platform for producing cellulolytic complex enzymes. However, their efficiency still requires enhancement to meet the cost-effective demands of lignocellulosic biomass conversion. Herein, secretome analysis revealed that the cellulolytic enzyme system of M. thermophila comprises the oxidative system consisting of lytic polysaccharide monooxygenases (LPMOs) and the hydrolytic system that includes endoglucanase, cellobiohydrolase, and beta-glucosidase. Both in vitro supplementation and in vivo overexpression of MtLPMOs with C1 or C1/C4 oxidizing activity enhanced the enzymatic saccharification of Avicel using M. thermophila fermentation broth, resulting in a maximum increase of 485% in oxidized cello-oligosaccharides production. Furthermore, the simultaneous enhancement of LPMO and beta-glucosidase expression in M. thermophila significantly improved cellulose depolymerization and lignocellulosic biomass degradation. Total production of native and oxidized cello-oligosaccharides from pretreated corncob residue increased from 5.55 to 0.27 mg/mL in the wild-type strain to 8.72 mg/mL and 0.61 mg/mL in the engineered strain. Taken together, these findings highlight the synergistic interaction between the oxidative and hydrolytic enzyme systems for efficient saccharification of lignocellulosic biomass, providing valuable insights for enhancing the performance of commercial cellulolytic enzyme products.

分类号：

  • 相关文献

[1]Discovering Functional Diversity of Lytic Polysaccharide Monooxygenases from the Thermophilic Fungus Myceliophthora Thermophila and Their Application in Lignocellulosic Biomass Degradation. Qin, Xing,Zou, Jiahuan,Yang, Kun,Li, Jinyang,Wang, Xiaolu,Tu, Tao,Zhang, Jie,Su, Xiaoyun,Yao, Bin,Huang, Huoqing,Luo, Huiying. 2023

[2]Development of an efficient protein expression system in the thermophilic fungus Myceliophthora thermophila. Jinyang Li,Yidi Wang,Kun Yang,Xiaolu Wang,Yuan Wang,Honglian Zhang,Huoqing Huang,Xiaoyun Su,Bin Yao,Huiying Luo,Xing Qin. 2023

[3]Heterologous expression and characterization of novel GH12 β-glucanase and AA10 lytic polysaccharide monooxygenase from Streptomyces megaspores and their synergistic action in cellulose saccharification. Xing Qin,Kun Yang,Jiahuan Zou,Xiaolu Wang,Tao Tu,Yuan Wang,Xiaoyun Su,Bin Yao,Huoqing Huang,Huiying Luo. 2023

[4]Insights into the H2O2-Driven Lytic Polysaccharide Monooxygenase Activity on Efficient Cellulose Degradation in the White Rot Fungus Irpex lacteus. Xing Qin,Kun Yang,Xiaolu Wang,Tao Tu,Yuan Wang,Jie Zhang,Xiaoyun Su,Bin Yao,Huoqing Huang,Huiying Luo. 2023

[5]G-lignin and hemicellulosic monosaccharides distinctively affect biomass digestibility in rapeseed. Pei, Yanjie,Li, Yuyang,Zhang, Youbing,Tu, Yuanyuan,Peng, Liangcai,Chen, Peng,Pei, Yanjie,Li, Yuyang,Zhang, Youbing,Fu, Tingdong,Zou, Jun,Tu, Yuanyuan,Peng, Liangcai,Chen, Peng,Pei, Yanjie,Li, Yuyang,Zhang, Youbing,Fu, Tingdong,Zou, Jun,Tu, Yuanyuan,Peng, Liangcai,Chen, Peng,Pei, Yanjie,Li, Yuyang,Zhang, Youbing,Fu, Tingdong,Zou, Jun,Tu, Yuanyuan,Peng, Liangcai,Chen, Peng,Yu, Changbing.

[6]Enzymatic saccharification of high pressure assist-alkali pretreated cotton stalk and structural characterization. Shuang-kui Du,Xia Su,Weihua Yang,Yanqin Wang,Meng Kuang,Lei Ma,Dan Fang,Dayun Zhou.

[7]White-rot fungi pretreatment combined with alkaline/oxidative pretreatment to improve enzymatic saccharification of industrial hemp. Xie, Chunliang,Gong, Wenbing,Yang, Qi,Zhu, Zuohua,Yan, Li,Hu, Zhenxiu,Peng, Yuande.

[8]Integrated pretreatment of poplar biomass employing p-toluenesulfonic acid catalyzed liquid hot water and short-time ball milling for complete conversion to xylooligosaccharides, glucose, and native-like lignin. Meysam Madadi,Dan Liu,Yuanhang Qin,Yinchao Zhang,Keikhosro Karimi,Meisam Tabatabaei,Vijai Kumar Gupta,Mortaza Aghbashlo,Zahoor,Sameh Samir Ali. 2023

[9]Sequential pretreatment with hydroxyl radical and manganese peroxidase for the efficient enzymatic saccharification of corn stover. Man Zhou,Yaru Wang,Yuan Wang,Tao Tu,Jie Zhang,Xiaolu Wang,Guijie Zhang,Huoqing Huang,Bin Yao,Huiying Luo,Xing Qin. 2024

[10]Biochemical and kinetic characterization of the multifunctional beta-glucosidase/beta-xylosidase/alpha-arabinosidase, Bgxa1. Gruninger, R. J.,Gong, X.,Forster, R. J.,McAllister, T. A.,Gong, X.. 2014

[11]Separation and Identification of Anthocyanins Extracted from Blueberry Wine Lees and Pigment Binding Properties toward beta-Glucosidase. Wu, Qian,Wang, Chao,Zhang, Yang,Chen, Yashu,Xie, Bijun,Sun, Zhida,Tang, Hu.

[12]Isolation of a maize beta-glucosidase gene promoter and characterization of its activity in transgenic tobacco. Gu, Riliang,Zhao, Li,Zhang, Ying,Chen, Xiaoping,Bao, Juan,Zhao, Jinfeng,Wang, Zhangying,Fu, Junjie,Liu, Tingsong,Wang, Jianhua,Wang, Guoying. 2006

[13]Overexpression of celB gene coding for beta-glucosidase from Pyrococcus furiosus using a baculovirus expression vector system in silkworm, Bombyx mori. Lin, Xu'Ai,Zhang, Wei,Chen, Yin,Bin Yao,Zhang, Zhi Fang.

[14]Engineering a highly active thermophilic beta-glucosidase to enhance its pH stability and saccharification performance. Xia, Wei,Shi, Pengjun,Bai, Yingguo,Luo, Huiying,Ma, Rui,Yao, Bin,Xia, Wei,Qian, Lichun,Xu, Xinxin. 2016

[15]Revisiting overexpression of a heterologous beta-glucosidase in Trichoderma reesei: fusion expression of the Neosartorya fischeri Bgl3A to cbh1 enhances the overall as well as individual cellulase activities. Xue, Xianli,Ma, Rui,Luo, Huiying,Su, Xiaoyun,Yao, Bin,Wu, Yilan,Qin, Xing. 2016

[16]Pretreatment of corn stover with acidic electrolyzed water and FeCl3 leads to enhanced enzymatic hydrolysis. Shen, Zhaobing,Jin, Chaonan,Shi, Jiping,Liu, Li,Shen, Zhaobing,Pei, Haisheng,Sun, Junshe,Sun, Junshe. 2014

[17]Exploring the cellulolytic and hemicellulolytic activities of manganese peroxidase for lignocellulose deconstruction. Xiaoqing Liu,Sunjia Ding,Fang Gao,Yaru Wang,Mohammad J. Taherzadeh,Yuan Wang,Xing Qin,Xiaolu Wang,Huiying Luo,Bin Yao,Huoqing Huang,Tao Tu. 2023

[18]Carbohydrate-binding modules facilitate the enzymatic hydrolysis of lignocellulosic biomass: Releasing reducing sugars and dissociative lignin available for producing biofuels and chemicals. Qicheng Shi,Ahmed M. Abdel-Hamid,Zhanying Sun,Yanfen Cheng,Tao Tu,Isaac Cann,Bin Yao,Weiyun Zhu. 2023

[19]Mo-modified MnOx for the efficient oxidation of high-concentration glucose to formic acid in water. Guo H.,Li J.,Xu S.,Yang J.,Chong G.-H.,Shen F.. 2023

[20]Bioenergy from dairy manure: technologies, challenges and opportunities. Qi-Li Zhu,Bo Wu,Nipon Pisutpaisal,Yan-Wei Wang,Ke-dong Ma,Li-Chun Dai,Han Qin,Fu-Rong Tan,Toshinari Maeda,Yan-sheng Xu,Guo-Quan Hu,Ming-Xiong He. 2021

作者其他论文 更多>>