数字农科院2.0

Porcine lung tissue slices: a culture model for PRCV infection and innate immune response investigations

文献类型： 外文期刊

作者： Shuxian Li;Yabin Lu;Shanshan Yang;Caiying Wang;Jing Yang;Xin Huang;Guohui Chen;Yongheng Shao;Maolin Li;Haoyuan Yu;Yuguang Fu;Guangliang Liu

作者机构：

关键词： Innate immune response;Porcine respiratory coronavirus;Precision-cut lung slices

期刊名称： AMB Express

ISSN： 2191-0855

年卷期： 2024 年 14 卷 1 期

页码：

收录情况： SCIE（2024版）

摘要： Respiratory coronaviruses (RCoVs) significantly threaten human health, necessitating the development of an ex vivo respiratory culture system for investigating RCoVs infection. Here, we successfully generated a porcine precision-cut lung slices (PCLSs) culture system, containing all resident lung cell types in their natural arrangement. Next, this culture system was inoculated with a porcine respiratory coronavirus (PRCV), exhibiting clinical features akin to humans who were infected by SARS-CoV-2. The results demonstrated that PRCV efficiently infected and replicated within PCLSs, targeting ciliated cells in the bronchioles, terminal bronchioles, respiratory bronchioles, and pulmonary alveoli. Additionally, through RNA-Seq analysis of the innate immune response in PCLSs following PRCV infection, expression levels of interferons, inflammatory cytokines and IFN stimulated genes were significantly upregulated. This ex vivo model may not only offer new insights into PRCV infection in the porcine respiratory tract but also serve as a valuable tool for studying human respiratory CoVs infection.

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