数字农科院2.0

CRISPR/Cas9-mediated gene editing of MsCLE3a confers compact alfalfa architecture

文献类型： 外文期刊

作者： Hefan Li;Cheng Li;García Caparrós Pedro;Qian Zhang;Hua Jiang;Yongping Yang;Yuanwen Duan;Xudong Sun

作者机构：

关键词： Cell wall;Leaf petiole angle;Medicago sativa;MsCLE3;Plant architecture

期刊名称： Plant Science

ISSN： 0168-9452

年卷期： 2025 年 357 卷

页码：

收录情况： SCIE（2025版）

摘要： Alfalfa (Medicago sativa L.) is a globally major forage crop for livestock due to its superior nutritional value and palatability. As a premium forage species, alfalfa breeding has traditionally emphasized high yield. In this study, we designed guide RNA (gRNA) to target the coding sequence of the MsCLE3a gene, and constructed a CRISPR/Cas9 vector to generate knockouts. Five mutant lines were successfully isolated, displaying upright petioles and a compact ternately compound leaf phenotype during the seedling stage. Cryo-scanning electron microscopy (Cryo-SEM) was employed to analyze cell morphology on the abaxial leaf surface. The results indicated that the cells on the abaxial side of the Mscle3a mutants were significantly elongated compared to the wild type (WT). Transcriptomic analysis further uncovered the down-regulation of brassinosteroid (BR) biosynthesis genes in Mscle3a mutants, which might underlie the mechanisms regulating petiole angle. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis revealed a major reduction in the transcript levels of several key genes involved in BR signaling pathways in the Mscle3a mutants relative to WT. This transcriptional down-regulation was strongly associated with the observed alterations in leaf petiole angle (LPA) in the mutant lines. Our findings provide novel insights into the novel regulatory role of MsCLE3a in alfalfa development and pinpoint potential targets for manipulating LPA in crop breeding programs.

分类号：

  • 相关文献

[1]Molecular cloning and characterization of a novel stress responsive gene in alfalfa. Sun, Y.,Long, R.,Yang, Q.,Kang, J.,Chao, Y.,Wu, M.,Long, R.,Wang, P.,Qin, Z.. 2012

[2]Effect of rhizobia symbiosis on lignin levels and forage quality in alfalfa (Medicago sativa L.). Zhang, Zhiqiang,Chang, Leqin,Cao, Yuman,Zhang, Tong,Wang, Yafang,Liu, Yushi,Zhang, Pan,Hu, Tianming,Yang, Peizhi,Zhang, Zhiqiang,Shao, Linhui,Zhang, Pan,Sun, Xiaoqin,Wu, Yajun.

[3]Expression of the alfalfa CCCH-type zinc finger protein gene MsZFN delays flowering time in transgenic Arabidopsis thaliana. Chao, Yuehui,Zhang, Tiejun,Yang, Qingchuan,Kang, Junmei,Qin, Zhihui,Sun, Yan,Gruber, Margaret Yvonne,Qin, Zhihui. 2014

[4]Expression of two uricase (Nodulin-35) genes in a non-ureide type legume, Medicago sativa. Cheng, XG,Nomura, M,Takane, K,Kouchi, H,Tajima, S. 2000

[5]Induction of heme oxygenase-1 with beta-CD-hemin complex mitigates cadmium-induced oxidative damage in the roots of Medicago sativa. Fu, Guangqing,Cui, Weiti,Wang, Yanqin,Shen, Wenbiao,Zhang, Liefeng,Ren, Yong,Zheng, Tianqing. 2011

[6]Molecular cloning and functional analysis of the drought tolerance gene MsHSP70 from alfalfa (Medicago sativa L.). Li, Zhenyi,Long, Ruicai,Zhang, Tiejun,Wang, Zhen,Zhang, Fan,Yang, Qingchuan,Kang, Junmei,Sun, Yan.

[7]Performance Evaluation Of A New Multifoliate Inbred Alfalfa (Medicago Sativa) Variety Via Space Mutation Breeding. Duan, Hui-Rong,Chai, Xiao-Qin,Yang, Hong-Shan,Zhou, Xue-Hui,Bao, Wen-Sheng,Chang, Gen-Zhu. 2019

[8]Completion of the agronomic evaluations of Medicago ruthenica [(L.) Ledebour] germplasm collected in Inner Mongolia. Campbell, TA,Bao, G,Xia, ZL. 1999

[9]Genetic relationships among alfalfa gemplasms resistant to common leaf spot and selected Chinese cultivars assessed by sequence-related amplified polymorphism (SARP) markers. Yuan, Qinghua,Wang, Yu,Li, Xiang-lin,Gao, Jianming,Gui, Zhi,Wang, Shuang,Zhao, Ximan,Xia, Buxian. 2011

[10]Molecular cloning and characterization of the MsHSP17.7 gene from Medicago sativa L.. Li, Zhen-yi,Long, Rui-cai,Zhang, Tie-jun,Yang, Qing-chuan,Kang, Jun-mei.

[11]Comparative Proteomic Analysis Reveals That A.ntioxidant System And Soluble S ugar Metabolism Contribute To Salt Tolerance In Alfalfa (Medicago Sativa L.) Leaves. Gao, YL, Long, RC, Rang, JM, Wang, Z, Zhang, TJ, Sun, H, Li, X, Yang, QC. 2019

[12]Melatonin Enhances Seed Germination and Seedling Growth of Medicago sativa Under Salinity via a Putative Melatonin Receptor MsPMTR1. Ruonan Yu,Tiantian Zuo,Pengfei Diao,Jiabin Fu,Yanyan Fan,Yue Wang,Qiqi Zhao,Xuesong Ma,Wenting Lu,Aoga Li,Ru Wang,Fang Yan,Li Pu,Yiding Niu,Hada Wuriyanghan. 2021

[13]The Conserved Cysteine-Rich Secretory Protein MaCFEM85 Interacts with MsWAK16 to Activate Plant Defenses. Ni Cai,Xiangqun Nong,Rong Liu,Mark Richard McNeill,Guangjun Wang,Zehua Zhang,Xiongbing Tu. 2023

[14]Accuracy of genomic selection for alfalfa biomass yield in two full-sib populations. Xiaofan He,Fan Zhang,Fei He,Yuhua Shen,Long Xi Yu,Tiejun Zhang,Junmei Kang. 2022

[15]How Fall Dormancy Benefits Alfalfa W.inter-Survival? Physiologic And Transcriptomic A nalyses Of Dormancy Process. Liu, ZY, Baoyin, TGT, Li, XL, Wang, ZL. 2019

[16]Overexpression of an ABA-inducible homeodomain-leucine zipper I gene MsHB7 confers salt stress sensitivity to alfalfa. Xiao Li,Yiyao Hou,Mingna Li,Fan Zhang,Fengyan Yi,Junmei Kang,Qingchuan Yang,Ruicai Long. 2022

[17]Fractal And Topological Analyses And Antioxidant Defense Systems Of Alfalfa (Medicago Sativa L.) Root System Under Drought And Rehydration Regimes. Li, S, Wan, LQ, Nie, ZN, Li, XL. 2020

[18]Nutritional quality and in vitro rumen fermentation characteristics of silage prepared with lucerne, sweet maize stalk, and their mixtures. Musen Wang,Fujin Zhang,Xinxin Zhang,Ying Yun,Lei Wang,Zhu Yu. 2021

[19]Genome-wide identification, phylogeny and expression analysis of the SPL gene family and its important role in salt stress in Medicago sativa L.. He, Fei,Long, Ruicai,Wei, Chunxue,Zhang, Yunxiu,Li, Mingna,Kang, Junmei,Yang, Qingchuan,Wang, Zhen,Chen, Lin. 2022

[20]Genome-Wide Identification and Characterization of Growth Regulatory Factor Family Genes in Medicago. Wenxuan Du,Junfeng Yang,Qian Li,Qian Su,Dengxia Yi,Yongzhen Pang. 2022

作者其他论文 更多>>