数字农科院2.0

The mining of thermostable β-glucosidase for tea aroma enhancement under brewing conditions

文献类型： 外文期刊

作者： Qi Lin;Cheng Peng;Kunpeng Yu;Yanling Lin;Yongquan Xu;Lijun Li;Hui Ni;Feng Chen

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关键词： Aroma enhancement;Glycosidic aroma compounds;Instant oolong tea;Thermostable β-glucosidase

期刊名称： Food Chemistry

ISSN： 0308-8146

年卷期： 2024 年 460 卷

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： The β-glucosidases known to improve tea aroma are all mesothermal enzymes, limiting their use under brewing conditions. Based on the properties analysis and molecular docking, the thermostable β-glucosidase (TPG) from Thermotoga petrophlia showed potential to enhance tea aroma. Treatment by recombinant TPG at 90 °C, the floral, sweet and grassy notes of instant Oolong tea were increased, while the roasted, caramel and woody notes were decreased. The improved floral, sweet and grassy notes were related to increase releasing of benzyl alcohol (floral), geraniol (floral), (Z)-3-hexen-1-ol (grassy), benzaldehyde (sweet) and 1-hexanol (grassy) by TPG hydrolyzing of (Z)-3-hexenyl-β-D-glucopyranoside, hexanyl-β-D-glucopyranoside (HGP), benzyl-β-D-glucopyranoside, prunasin and geranyl-β-D-glucopyranoside (GGP), respectively. Although the catalytic efficiency of TGP to GGP was about twice that to HGP, HPG was more competitive than GGP when they mixed. Combined with microstructure analysis, the structure-function relationship of TPG-influencing tea aroma were understood. This study provided the method of how to mining new function of characterized β-glucosidases, as well as a theoretical basis for the development of new tea products.

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