数字农科院2.0

Optimal harvest period and quality control markers of cultivated Flos Chrysanthemi Indici using untargeted/targeted metabolomics, chemometric analysis and in vivo study

文献类型： 外文期刊

作者： Peng Yang;Dong Tian;Xiao Yu Han;Qing Jun Zou;Liang Ju Ma;Min Wei;Meng Yu;Zhong Mei Zou

作者机构：

关键词： 3,5-Dicaffeoylquinic acid;Apigenin;Flos Chrysanthemi Indici;Linarin;Luteolin;Optimal harvest period

期刊名称： Journal of Ethnopharmacology

ISSN： 0378-8741

年卷期： 2024 年 334 卷

页码：

收录情况： SCIE（2024版）

摘要： Ethnopharmacological relevance: Flos Chrysanthemi Indici (FCI), the flower of Chrysanthemum Indicum L., is a popular traditional Chinese medicine (TCM) for treatment of inflammatory diseases in China. FCI is also a functional food, and is widely used as herbal tea for clearing heat and detoxicating. Aim of the study: To explore quality control markers of FCI based on the optimal harvest period. Materials and methods: First, UPLC-Q-TOF/MS based untargeted metabolomics was applied to explore the chemical profiles of FCIs collected at bud stages (BS), initial stages (IS), full bloom stages (FS) and eventual stages (ES) from eight cultivated regions in China. Subsequently, lipopolysaccharide (LPS)-induced RAW264.7 cell inflammatory model and carrageenan-induced rat paw edema model were used to confirm the anti-inflammatory effect of FCIs collected at IS/FS. Then, UPLC-PDA targeted metabolomics was used to quantitatively analyze 9 constituents with anti-inflammatory activity (7 flavonoids and 2 phenolic acids) changed significantly (VIP > 4) during flowering stages. Finally, ROC curves combined with PCA analysis based on the variation of 9 active constituents in FCIs from different flowering stages were applied to screen the quality markers of FCI. Results: FCIs at IS/FS had almost same chemical characteristics, but quite different from those at BS and ES. A total of 32 constituents in FCIs including flavonoids and phenolic acids were changed during flowering development. Most of the varied constituents had the highest or higher contents at IS/FS compared with those at ES, indicating that the optimal harvest period of FCI should be at IS/FS. FCI extract could effectively suppress nitric oxide (NO) production in LPS-induced RAW264.7 cells and regulate the abnormal levels of cytokines and PGE2 in carrageenan-induced paw edema model rat. The results of quantitatively analysis revealed that the variation trends of phenolic acids and flavonoids in FCIs were different during flowering development, but most of them had higher contents at IS/FS than those at ES in all FCIs collected from eight cultivated regions, except one sample from Anhui. Finally, linarin, luteolin, apigenin and 3,5-dicaffeoylquinic acid were selected as the Q-markers based on the contribution of their AUC values in ROC and clustering of PCA analysis. Conclusions: Our study demonstrates the optimal harvest period of FCI and specifies the multi-constituents Q-markers of FCI based on the influence of growth progression on the active constituents using untargeted/targeted metabolomics. The findings not only greatly increase the utilization rate of FCI resources and improve quality control of FCI products, but also offer new strategy to identify the Q-markers of FCI.

分类号：

  • 相关文献

[1]Variation In Chemical Composition And Biological Activities Of Flos Chrysanthemi Indici Essential Oil Under Different Extraction Methods. Jing, CL, Huang, RH, Su, Y, Li, YQ, Zhang, CS. 2019

[2]Enhanced Anti-Inflammatory Activities by the Combination of Luteolin and Tangeretin. Funaro, Antonietta,Wu, Xian,Song, Mingyue,Zheng, Jinkai,Guo, Shanshan,Rakariyatham, Kanyasiri,Xiao, Hang,Funaro, Antonietta,Rodriguez-Estrada, Maria Teresa,Xiao, Hang,Zheng, Jinkai,Guo, Shanshan. 2016

[3]Antiviral Activity of Luteolin against Pseudorabies Virus In Vitro and In Vivo. Xiaoyu Men,Su Li,Xiaojing Cai,Lian Fu,Yi Shao,Yan Zhu. 2023

[4]Highly pathogenic porcine reproductive and respiratory syndrome virus-induced inflammatory response in porcine pulmonary microvascular endothelial cells and effects of herbal ingredients on main inflammatory molecules. Wu Y.,Song X.,Li P.,Wang Z.,Zhao Z.,Zhang T.. 2023

[5]Plant exosome-like nanovesicles derived from sesame leaves as carriers for luteolin delivery: Molecular docking, stability and bioactivity. Jiang, Dan,Li, Ziliang,Liu, Hongyan,Liu, Huihui,Xia, Xiaoyang,Xiang, Xia. 2024

[6]Exploring synergistic inhibitory mechanisms of flavonoid mixtures on α-glucosidase by experimental analysis and molecular dynamics simulation. Pan, Junkun,Nawaz, Muhammad,Liu, Jiechao,Liu, Hui,Lv, Zhenzhen,Yang, Wenbo,Jiao, Zhonggao,Zhang, Qiang. 2024

[7]A Pectin-Based Active Coating for Preservation of Fresh-Cut Apples: Incorporated with Luteolin and ε-Polylysine for Enhanced Performance. Li, Chengheng,Pan, Junkun,Nawaz, Muhammad,Liu, Hui,Lv, Zhenzhen,Yang, Wenbo,Zhang, Qiang,Liu, Jiechao,Jiao, Zhonggao. 2025

[8]The effects of flavonoid apigenin on male reproductive health: Inhibition of spermatogonial proliferation through downregulation of Prmt7/AKT3 pathway. Bingyuan Wang,Mingrui Zhang,Jiankang Guo,Zhiguo Liu,Rong Zhou,Fei Guo,Kui Li,Yulian Mu. 2021

[9]Protective Effects Of Five Structurally D.iverse Flavonoid Subgroups Against C hronic Alcohol-Induced Hepatic Damage In A Mouse Model. Zhao, L, Zhang, NH, Yang, D, Yang, MY, Guo, XX, He, JG, Wu, W, Ji, BP, Cheng, Q, Zhou, F. 2018

[10]The effects of flavonoid apigenin on male reproductive health: Inhibition of spermatogonial proliferation through downregulation of Prmt7/AKT3 pathway. Bingyuan Wang,Mingrui Zhang,Jiankang Guo,Zhiguo Liu,Rong Zhou,Fei Guo,Kui Li,Yulian Mu. 2021

[11]The haplotype-resolved genome of diploid Chrysanthemum indicum unveils new acacetin synthases genes and their evolutionary history. Hou, Zhuangwei,Yang, Song,He, Weijun,Lu, Tingting,Feng, Xunmeng,Zang, Lanlan,Bai, Wenhui,Chen, Xueqing,Nie, Bao,Li, Cheng,Wei, Min,Ma, Liangju,Han, Zhengzhou,Zou, Qingjun,Li, Wei,Wang, Li. 2024

[12]The roles of flaxseed gum and its oligosaccharides as stabilizers in zein nanoparticles for apigenin delivery: Stability, antioxidant activity, bioavailability, molecular simulations. Yufei Liu,Ziliang Li,Shuaijun Lu,Shang Gao,Mengting Xu,Ze Yuan,Yue Li,Yiqiao Gao,Jingfang Shangguan,Xia Xiang. 2025

[13]Apigenin Ameliorates Insulin Resistance in 3T3-L1 Adipocytes: Establishment of a New Insulin Resistance Model Induced by Combined Treatments. Guo, Xiaoxuan,Xia, Bing,Liu, Sha,Dong, Ying,Qian, Yongzhong,Qiu, Jing. 2025

[14]pH-driven plant protein nanoparticles modulated by flaxseed gum oligosaccharides for apigenin delivery . Ziliang Li,,Yufei Liu,,Shuaijun Lu ,,Shang Gao ,, Li Chen ,, Qianchun Deng,, Zhenxia Xu,,Xia Xiang. 2025

[15]Apigenin Alleviates Zearalenone-Induced Oxidative Stress and Apoptosis in Swine Testis Cells Through the Wnt Signaling Pathway. Guo, Chenyun,Zhang, Yidan,Wang, Yiwei,Sun, Yile,Ning, Haoze,Gao, Jiaxin,Guo, Fei,Ji, Pengyun,Zhang, Lu,Liu, Guoshi,Wang, Bingyuan. 2025

作者其他论文 更多>>