数字农科院2.0

Exploring the potential role of EPSPS mutations for enhanced glyphosate resistance in Nicotiana tabacum

文献类型: 外文期刊

作者: Bingjie Li;Chen Chen;Mengmeng Cui;Yuhe Sun;Jing Lv;Changbo Dai

作者机构:

关键词: EPSP synthase;gene editing;glyphosate;herbicide resistance;tobacco

期刊名称: Frontiers in Plant Science

ISSN: 1664-462X

年卷期: 2025 年 16 卷

页码:

收录情况: SCIE(2025版)

摘要: Glyphosate is a widely used non-selective, broad-spectrum, systemic herbicide by interfering with the biosynthesis of aromatic amino acids. However, the emergence of glyphosate-resistant weeds has driven the need for enhanced herbicide resistance in crops. In this study, we engineered two mutant variants of the tobacco EPSPS gene through amino acid substitution (TIPS-NtEPSPS and P180S-NtEPSPS). These mutated EPSPS genes were overexpressed in tobacco under the control of CaMV35S promoters. Our results demonstrate that overexpression of TIPS-NtEPSPS significantly enhances glyphosate tolerance, allowing plants to withstand up to four times the recommended dose without compromising their fitness. This research highlights the potential of the TIPS-NtEPSPS mutant to improve herbicide resistance in tobacco, offering a viable approach for effective weed management.

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