数字农科院2.0

Pharmacological activation of Wnt/β-catenin signaling is essential for oocyte developmental competence and enhances in vitro embryo production in water buffalo

文献类型： 外文期刊

作者： Ma, Xiaoya;Zheng, Haiying;Duan, Anqin;Xie, Yingxue;Zhou, Jinchen;Shang, Jianghua;Yang, Chunyan

作者机构：

关键词： Wnt beta-catenin signaling;Buffalo folliculogenesis;Follicular selection;Granulosa cells;Steroidogenesis

期刊名称： ANIMAL REPRODUCTION SCIENCE

ISSN： 0378-4320

年卷期： 2025 年 285 卷

页码：

收录情况： SCIE（2025版）

摘要： The Wnt/(3-catenin signaling pathway is integral to follicular development, yet its precise role in buffalo folliculogenesis remains poorly defined. This study aimed to elucidate its involvement in follicular selection and underlying regulatory mechanisms. Buffalo follicles were classified as dominant or atretic based on the follicular fluid estradiol-to-progesterone (E2: P4) ratio. Quantitative PCR revealed significantly elevated expression of Wnt/(3-catenin pathway components in the granulosa cells of dominant follicles. Functional experiments demonstrated that pharmacological inhibition of Wnt/(3-catenin signaling markedly suppressed both FSH-induced estradiol (E2) production and oocyte maturation. Conversely, Wnt/(3-catenin pathway activation enhanced oocyte maturation, cleavage, and blastocyst formation rates. Further investigation identified AKT/GSK3(3/(3-catenin cascade as a key mediator of Wnt's positive regulatory role in FSHsupported steroidogenesis. These findings clarify the pivotal role of Wnt/(3-catenin signaling in buffalo follicular selection and provide a foundational strategy for enhancing the efficiency of in vitro embryo production (IVP) in buffalo breeding programs.

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