数字农科院2.0

Establishment of Interspecies Somatic Cell Nuclear Transfer and Transgene-Free Inducible Pluripotent Stem Cells for Versatile Conservation of the Germplasm Resource of Wild Boar

文献类型： 外文期刊

作者： Gao, Chen;Zhou, Xinyi;Gu, Shigang;Li, Jianbo;Wang, Jing;Zhang, Yurong;Zhao, Wenxuan;Wei, Xinhua;Lu, Liting;Zhao, Quanmin;Huang, Yongye;Yu, Dawei

作者机构：

关键词： germplasm resource;pig;reprogramming;somatic cell nuclear transfer;stem cells

期刊名称： ANIMAL RESEARCH AND ONE HEALTH

ISSN： 2835-5075

年卷期： 2025 年

页码：

收录情况： 无来源刊（2025版）

摘要： Cloning via somatic cell nuclear transfer and establishing inducible pluripotent stem cells is critical for the conservation of the cherished germplasm resource. The wild boar population would be altered due to human activities and climate change. Recently, we have successfully generated cloned wild boars via interspecies somatic cell nuclear transfer using wild boar fibroblast cells as donor cells, domestic porcine oocytes as recipients, and Bama pigs as surrogate sows. We have also harvested wild boar inducible pluripotent stem cells free of selection genes utilizing the piggyBac transposon system. This study sheds light on preserving the genetic resource of wild boar and provides a reference for protecting other wildlife.

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