数字农科院2.0

Detection Of Circulating Cell-Free Dna To Diagnose Schistosoma Japonicum Infection

文献类型： 外文期刊

作者： Ullah, H; Qadeer, A; Giri, BR

作者机构：

关键词： Schistosoma Japonicum; Molecular Diagnosis; Cell-Free Dna; Internal Transcribed Spacer 2

期刊名称： ACTA TROPICA

ISSN： 0001-706X

年卷期： 2020 年 211 卷

页码：

收录情况： JCR（2021版）

摘要： Schistosomiasis occurs in 240 million people worldwide and is a major public health concern. Thus, early diagnosis and monitoring of schistosomiasis progression are needed to treat patients. Cell-free DNA (cfDNA) is present as fragments of parasite-derived DNA in host body fluids. Detection of this cfDNA in host blood may be a promising diagnostic marker of schistosomiasis. Therefore, in this study, we investigated the potential of internal transcribed spacer 2 (ITS2), a molecular taxonomy and barcoding marker, in diagnosing schistosomiasis using infected rabbit and mice sera. A 192 bp fragment of ITS2 was detected in the serum-isolated DNA from the infected host on different days after infection. We also determined the sensitivity of detecting ITS2 in mice with varying numbers of cercaria: cfDNA was present even in mice with low abundance of the parasite. Overall, our results show that cfDNA may be a potential tool for the early diagnosis and therapeutic evaluation of S. japonicum infection.

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