数字农科院2.0

Nanopore sequencing: flourishing in its teenage years

文献类型： 外文期刊

作者： Zhang, Tianyuan;Li, Hanzhou;Jiang, Mian;Hou, Huiyu;Gao, Yunyun;Li, Yali;Wang, Fuhao;Wang, Jun;Peng, Kai;Liu, Yong-Xin

作者机构：

关键词： （1-1-1）Nanopore sequencing;Long-read sequencing;Application;Accuracy;Outlook;Direct RNA sequencing;Metagenomics

期刊名称： JOURNAL OF GENETICS AND GENOMICS

ISSN： 1673-8527

年卷期： 2025 年 51 卷 12 期

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版）

摘要： Over the past decade, nanopore sequencing has experienced significant advancements and changes, transitioning from an initially emerging technology to a significant instrument in the field of genomic sequencing. However, as advancements in next-generation sequencing technology persist, nanopore sequencing also improves. This paper reviews the developments, applications, and outlook on nanopore sequencing technology. Currently, nanopore sequencing supports both DNA and RNA sequencing, making it widely applicable in areas such as telomere-to-telomere (T2T) genome assembly, direct RNA sequencing (DRS), and metagenomics. The openness and versatility of nanopore sequencing have established it as a preferred option for an increasing number of research teams, signaling a transformative influence on life science research. As the nanopore sequencing technology advances, it provides a faster, more costeffective approach with extended read lengths, demonstrating the significant potential for complex genome assembly, pathogen detection, environmental monitoring, and human disease research, offering a fresh perspective in sequencing technologies. Copyright (c) 2024, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights are reserved,

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