数字农科院2.0

Development of an on-site diagnostic LAMP assay for rapid differentiation of the invasive pest Phthorimaea absoluta (Meyrick) using insect tissues

文献类型： 外文期刊

作者： Yang, Li-Feng;Liu, Ya-Ge;Tao, Yun-Li;Zhang, Wan-Min;Li, Jian-Yong;Chi, Sheng-Qi;Zhang, Gui-Fen;Chu, Dong

作者机构：

关键词： loop mediated isothermal amplification (LAMP);Phthorimaea absoluta;invasive species;on-site diagnostics

期刊名称： PEST MANAGEMENT SCIENCE

ISSN： 1526-498X

年卷期： 2024 年

页码：

收录情况： SCIE（2024版）

摘要： BACKGROUND The tomato leafminer, Phthorimaea absoluta (Meyrick) (Lepidoptera: Gelechiidae), is a destructive invasive pest that originated in South America and has spread within China since 2017. A rapid method for on-site identification of P. absoluta is urgently needed for interception of this pest across China. RESULTS We developed a loop-mediated isothermal amplification (LAMP) technique to differentiate P. absoluta from Liriomyza sativae, Chromatomyia horticola, and Phthorimaea operculella using extracted genomic DNA, which was then refined to create an on-site LAMP diagnostic method that can be performed under field conditions without the need for laboratory equipment. CONCLUSION In the present research, we developed an on-site diagnostic method for rapid differentiation of P. absoluta from other insects with similar morphology or damage characteristics in China.

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