数字农科院2.0

Expression and characterization of canine distemper virus hemagglutinin protein in suspension mammalian cells

文献类型： 外文期刊

作者： Cai, Jiaxi;Li, Zishu;Wang, Yu;Fang, Shuren;Fang, Xiaohan;Xue, Xianghong

作者机构：

关键词： Canine distemper virus;Hemagglutinin protein;Secreted expression;Immunogenicity

期刊名称： JOURNAL OF VIROLOGICAL METHODS

ISSN： 0166-0934

年卷期： 2025 年 333 卷

页码：

收录情况： SCIE（2025版）

摘要： Hundreds of millions of the domestic dogs worldwide are routinely inoculated with the modified live vaccines for canine distemper virus (CDV) every year. However, the corresponding serological diagnostic and detections are always lacking, thus, there is an urgent demand to establish its unique diagnostic technologies to produce highquality antigenic biomolecules. In the present study, the ectodomain (et) of CDV hemagglutinin (H) protein was firstly expressed in a soluble and secreted forms by an Expi293F transient transfection system based on its antigenic secondary structure analysis. The yields of CDV H(et) protein was 2.6 g/L with purity over 95 % in supernatant of Expi293F cells. The CDV H(et) protein elicited comparative antibodies levels to the CDV virions in rabbit by ELISA and neutralization test. The purified polyclonal antibodies of immunized with CDV H(et) protein recognized both wild-type and vaccine CDV strains. More importantly, the purified polyclonal antibodies of CDV H(et) protein revealed significantly higher viral neutralizing activity than those from CDV-3 virions, which highlighted that the critical role of CDV H protein to elicit viral specific and protective neutralizing antibodies. Taken together, the CDV H(et) protein produced in mammalian expression systems was high-quality and good immunogenicity, and would be with great potential to serve as a serological diagnostic antigen or a novel CDV subunit vaccine in future.

分类号：

  • 相关文献

[1]Screening signal peptides from Bacillus thuringiensis secretome for heterologous protein secretion. Jianbo Yang,Yan Xia,Li Zhu,Zhiwen Wang,Fuping Song,Shuyuan Guo. 2025

[2]Recombinant adenovirus expressing F and H fusion proteins of peste des petits ruminants virus induces both humoral and cell-mediated immune responses in goats. Wang, Yong,Xu, Binrui,Wang, Yong,Shi, Lijun,Li, Wenchao,Huang, Huaxin,Tao, Chunai,Cheng, Chaofei,Li, Gang,Liu, Guangqing,Chen, Zongyan,Li, Chuanfeng. 2013

[3]Recombinant Marek's disease virus type 1 provides full protection against H9N2 influenza A virus in chickens. Yuntong Chen,Qingqing Yu,Wenrui Fan,Xianying Zeng,Zibo Zhang,Guobin Tian,Changjun Liu,Hongmei Bao,Longbo Wu,Yanping Zhang,Yongzhen Liu,Suyan Wang,Hongyu Cui,Yulu Duan,Hualan Chen,Yulong Gao. 2024

[4]Development and characterization of a novel nanobody with SRMV neutralizing activity. Miao Sun,Changjiang Wang,Huaye Luo,Yanfei Chen,Guanggang Qu,Jian Chen,Ling Li,Min Zhang,Qinghong Xue. 2024

[5]Characterization of a new dog isolate of canine distemper virus from China. Qiao, J.,Meng, Q.,Chen, C.,Xia, X.,Ren, Y.,Zhang, H.,Qiao, J.,Cai, X.. 2011

[6]Phylogenetic analysis of the haemagglutinin gene of canine distemper virus strains detected from breeding foxes, raccoon dogs and minks in China. Zhao, Jian-Jun,Yan, Xi-Jun,Chai, Xiu-Li,Luo, Guo-Liang,Zhang, Hai-Ling,Gao, Han,Bai, Xue,Zhang, Lei,Chen, Tao,Xu, Lei,Zhao, Chun-Fei,Wang, Feng-Xue,Shao, Xi-Qun,Wu, Wei,Cheng, Shi-Peng,Martella, Vito,Liu, Ying-Xue.

[7]Recombinant rabies virus expressing the H protein of canine distemper virus protects dogs from the lethal distemper challenge. Wang, Feng-Xue,Zhang, Shu-Qin,Zhu, Hong-Wei,Yang, Yong,Sun, Na,Tan, Bin,Li, Zhen-Guang,Cheng, Shi-Peng,Wen, Yong-Jun,Li, Zhen-Guang,Fu, Zhen F..

[8]Development of a combined canine distemper virus specific RT-PCR protocol for the differentiation of infected and vaccinated animals (DIVA) and genetic characterization of the hemagglutinin gene of seven Chinese strains demonstrated in dogs. Yi, Li,Cheng, Shipeng,Xu, Hongli,Wang, Jianke,Cheng, Yuening,Yang, Shen,Luo, Bin. 2012

[9]Inactivated Recombinant Rabies Viruses Displaying Canine Distemper Virus Glycoproteins Induce Protective Immunity against Both Pathogens. Budaszewski, Renata da Fontoura,Budaszewski, Renata da Fontoura,Sawatsky, Bevan,Kraemer, Beate,von Messling, Veronika,Sawatsky, Bevan,von Messling, Veronika,Hudacek, Andrew,Yin, Xiangping,Schnell, Matthias J.,Yin, Xiangping.

[10]Identification of a novel canine distemper virus B-cell epitope using a monoclonal antibody against nucleocapsid protein. Yi, Li,Cheng, Yuening,Zhang, Miao,Cao, Zhigang,Tong, Mingwei,Wang, Jianke,Zhao, Hang,Lin, Peng,Cheng, Shipeng.

[11]Recombinant Newcastle disease viral vector expressing hemagglutinin or fusion of canine distemper virus is safe and immunogenic in minks. Ge, Jinying,Wang, Xijun,Tian, Meijie,Wen, Zhiyuan,Yu, Guimei,Zhou, Weiwei,Zu, Shulong,Bu, Zhigao,Gao, Yuwei.

[12]Pathogenesis of canine distemper virus in experimentally infected raccoon dogs, foxes, and minks. Zhao, Jianjun,Shi, Ning,Sun, Yangang,Zhu, Chunsheng,Zhang, Hailing,Hu, Bo,Bai, Xue,Yan, Xijun,Zhao, Jianjun,Yan, Xijun,Martella, Vito,Nikolin, Veljko.

[13]Isolation and sequence analysis of a canine distemper virus from a raccoon dog in Jilin Province, China. Cheng, Yuening,Wang, Jianke,Zhang, Miao,Zhao, Jianjun,Shao, Xiqun,Zhao, Hang,Lin, Peng,Wu, Hua,Ma, Zengjun.

[14]Emergence of canine distemper virus strains with two amino acid substitutions in the haemagglutinin protein, detected from vaccinated carnivores in North-Eastern China in 2012-2013. Zhao, Jianjun,Zhang, Hailing,Bai, Xue,Hu, Bo,Sun, Yangang,Zhu, Chunsheng,Zhang, Lei,Liu, Hao,Xu, Shujuan,Shao, Xiqun,Wu, Wei,Yan, Xijun,Zhao, Jianjun,Yan, Xijun,Martella, Vito. 2014

[15]Phylodynamic Analysis Of Two Amino Acid Substitutions In The Hemagglutinin Protein Of Canine Distemper Virus Strains Detected In Fur-Bearing Animals In China. Tao, RS, Ba, HX, Chen, J, Liu, MJ, Pan, HJ, Li, X, Wang, QK, Zhao, JJ. 2020

[16]DNA Vaccine Co-Expressing Hemagglutinin and IFN-γ Provides Partial Protection to Ferrets against Lethal Challenge with Canine Distemper Virus. Jianjun Zhao,Yiyang Sun,Ping Sui,Hongjun Pan,Yijun Shi,Jie Chen,Hailing Zhang,Xiaolong Wang,Rongshan Tao,Mengjia Liu,Dongbo Sun,Jiasan Zheng. 2023

[17]Persistent and Severe Viral Replication in PBMCs with Moderate Immunosuppression Served an Alternative Novel Pathogenic Mechanism for Canine Morbillivirus. Feng C.,Bu Y.,Cai J.,Zhao G.,Li Z.,Cheng Y.,Zhang X.,Shi Y.,Gao Y.,Li X.,Zheng X.,Xue X.. 2023

[18]Multiple Receptors Involved in Invasion and Neuropathogenicity of Canine Distemper Virus: A Review. Jianjun Zhao,Yanrong Ren. 2022

[19]Viral Pathogenesis, Recombinant Vaccines, And Oncolytic Virotherapy: Applications Of The Canine Distemper Virus Reverse Genetics System. Zhao, JJ, Ren, YR, Chen, J, Zheng, JS, Sun, DB. 2020

[20]Identification Of Linear B-Cell Epitopes O.n The Phosphoprotein Of C anine Distemper Virus Using Four Monoclonal Antibodies. Li, S, Yi, L, Cao, ZG, Cheng, YN, Tong, MW, Wang, JK, Lin, PK, Cheng, SP. 2018

作者其他论文 更多>>