数字农科院2.0

Genetic characteristics and pathogenicity of variant porcine parvovirus 1 in northern China

文献类型： 外文期刊

作者： Huiwei Deng;Mengting Wang;Guangyi Cong;Fang Fu;Li Feng

作者机构：

关键词： Neutralization;Pathogenicity;Porcine parvovirus;PPV;Sequence analysis

期刊名称： Veterinary Microbiology

ISSN： 0378-1135

年卷期： 2024 年 298 卷

页码：

收录情况： SCIE（2024版）

摘要： Porcine parvovirus type 1 (PPV1) can lead to reproductive disorders in pregnant sows, including stillbirth, mummification, embryonic death, and infertility (SMEDI syndrome). In this study, we isolated and identified 10 PPV1 strains in northern China, with genomes around 5 kb long and minor deletions in the 127-nt repeat region. The sequence analysis results showed that compared with strain NADL-2 (Reference strain), eight amino acid substitutions on the NS1 protein and fourteen amino acid substitutions on the VP2 protein were found in the ten isolates. Because the JX strain exhibited reduced neutralizing activity induced by commercially available vaccines in vitro, it was selected for challenge experiments in sows at 35 days of gestation. Mutant strain JX not only caused viremia, but also mild edema and mild inflammation in the trachea, lungs, lymph nodes, reproductive organs, and some intestines of the pregnant sows. Strain JX also caused fetal congestion and organ infection after penetrating the placental barrier. In conclusion, this study focused on the variation and evolution of PPV1 in northern China, screened a strain with reduced neutralizing activity, and determined that it has a certain degree of pathogenicity.

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