数字农科院2.0

Unveiling the role of N-doped carbon quantum dots in relieving humic acid inhibition and promoting methanogenesis via enhanced electron transfer

文献类型： 外文期刊

作者： Qiang Liu;Mahmoud Mazarji;Yeqing Li;Lu Feng;Hongjun Zhou;Quan Xu;Alastair James Ward;Yajing Wang;Jianping Su;Chunming Xu;Junting Pan

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关键词： 13C NMR 13C Nuclear Magnetic Resonance Spectra;Abbreviations AD Anaerobic digestion;AFM Atomic force microscope;AS-HA HA extracted from activated sludge;ATP Adenosine triphosphate;BLI Biolayer interferometry;BMP Biochemical methane potential;CDD Charge density difference;CLSM Confocal laser scanning microscope;CQD Carbon quantum dots;CV Cyclic voltammetry;D-value Difference value;DET Direct electron transfer;DFT Density functional theory;DI Deionized water;DIET Direct interspecies electron transfer;ECSA Electrochemical active surface area;EEM Excitation-Emission-Matrix Spectra;EET extracellular electron transfer;ETSA Electric transport system activity;FRET Förster resonance energy transfer;FTIR Flight Test Instrumentation Requirements;Glu Glucose;Gra-N graphite-N;HA Humic acid;HI/HB Hydrophilicity/Hydrophobicity;ID/IGD peak intensity/G peak intensity;Ino inoculum sludge;KDdissociation constant;kdisdisassociation rate constant;konassociation rate constant

期刊名称： Journal of Environmental Chemical Engineering

ISSN： 2213-3437

年卷期： 2025 年 13 卷 5 期

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Humic acid (HA) is an inhibitor that can diminish bioconversion during anaerobic digestion (AD). In this study, a non-metallic nanomaterial, N-doped carbon quantum dots (NCQD), was synthesized to alleviate the HA inhibition, followed by exploring its mechanism. Adding 500mg/L NCQD prevented HA inhibition by binding to HA or microorganism surfaces, thus avoiding contact between them. This phenomenon, known as shielding inhibition, involves the formation of hydrogen bonds and chemical bonds. The intermolecular force and dissociation constant (KD) between NCQD and HA were determined to be 112.83±3.98 nN and 1.0±0.07×10-7 M, respectively. NCQD could promote electron transfer in AD systems. According to the in-depth microbial analysis, NCQD significantly enhanced the metabolic pathways of methanogenesis and biosynthesis of coenzyme F420. This groundbreaking research demonstrates the dual functionality of NCQD by effectively shielding against HA inhibition and promoting electron transfer. The study also unravels the mechanism of interface interaction, electron transfer, and metabolic pathways, leading to significant breakthroughs in addressing HA inhibition.

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