数字农科院2.0

GhJOX2, a jasmonic acid oxidase, modulates cotton's resistance to Verticillium wilt through interaction with GhSAMS1

文献类型： 外文期刊

作者： Pang, Chenxi;Zhao, Pei;Sang, Xiaohui;Chen, Wei;Wang, Hongmei;Zhao, Yunlei

作者机构：

关键词： Cotton and Verticillium wilt andGhJOX2and JA Signaling Pathway

期刊名称： INDUSTRIAL CROPS AND PRODUCTS

ISSN： 0926-6690

年卷期： 2025 年 239 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Verticillium wilt (VW) severely impacts cotton production, and it is essential to detect genes related to VW resistance and develop resistant varieties with genetic engineering in cotton. Jasmonic acid (JA) induces broadspectrum disease resistance, while its overaccumulation hinders plant growth. However, JA can be degraded by Jasmonate-induced oxygenases (JOXs) which hydroxylating it into 12-OH-JA, balancing defense and growth. In previous research, a jasmonate oxidase gene, GhJOX2, was identified to be involved in Verticillium wilt resistance in cotton. Loss-of-function of this gene in cotton enhanced the disease resistance. In this study, we further demonstrated that GhJOX2-silenced plants showed significantly elevated JA content upon pathogen challenge, and overexpression of GhJOX2 enhanced the susceptibility of Arabidopsis thaliana to Verticillium dahliae infection, downregulating the expression of JA pathway-related genes. GhJOX2 was found to interact with GhSAMS1 encoding S-adenosylmethionine synthase associated with ethylene biosynthesis, and silencing GhSAMS1 enhanced cotton resistance against Verticillium dahliae and activated multiple defense responses including reactive oxygen species (ROS) accumulation and defense gene upregulation. It is speculated that GhJOX2 negatively regulates cotton's resistance to Verticillium wilt through hydroxylating jasmonic acid and interacting with the GhSAMS1.

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