数字农科院2.0

Eimeria tenella apical membrane 2 overexpression enhances pathogenicity and immunity in chickens

文献类型： 外文期刊

作者： Wu, Shanbo;Wang, Lihui;Wang, Jinwen;Han, Wenqi;Bai, Jiayu;Zhao, Fanghe;Yu, Yu;Zhao, Qiping;Zhu, Shunhai;Han, Hongyu;Dong, Hui

作者机构：

关键词： Eimeria tenella;EtAMA2;Overexpression;Virulence;Immunogenicity

期刊名称： PARASITES & VECTORS

ISSN： 1756-3305

年卷期： 2025 年 19 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： BackgroundEimeria tenella, a highly pathogenic apicomplexan parasite, causes severe avian coccidiosis, threatening global poultry production. Apical membrane antigens (AMAs), conserved proteins in apicomplexan parasites, are critical for host cell invasion, making them promising vaccine targets. In this study, we constructed an the overexpression strain of E. tenella AMA2 (EtAMA2-OE) and evaluated its pathogenicity and immunogenicity.MethodsTranscriptional profiling of EtAMA2 during infection was conducted using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). A homozygous EtAMA2-OE strain was generated using plasmid transfection, drug selection, and flow cytometry. Pathogenicity was assessed through in vitro sporozoite invasion assays and in vivo evaluations, including cecal lesion scoring, oocyst shedding, and weigh-gain monitoring in chicks. Furthermore, immunogenicity was evaluated by challenging immunized chicks with wild-type E. tenella.ResultsThe EtAMA2-OE strain significantly enhanced invasion efficiency and pathogenicity, causing severe cecal pathology, increased oocyst output, and weight loss. Importantly, immunization with EtAMA2-OE conferred substantial immunity, resulting in significantly reduced oocyst shedding and markedly attenuated cecal lesions in immunized chicks.ConclusionsThe present data indicated that EtAMA2 had dual functions as a virulence factor critical for early invasion and a promising vaccine antigen. This study thereby provided new insights for future drug target screening and development of a vaccine against coccidiosis.

分类号：

  • 相关文献

[1]EtMIC2 overexpression attenuates pathogenicity while enhancing oocyst production and immunogenicity in Eimeria tenella. Yu Qiao,Jinwen Wang,Qiping Zhao,Shunhai Zhu,Yu Yu,Shanbo Wu,Fanghe Zhao,Wenqi Han,Jiayu Bai,Hongyu Han,Hui Dong. 2025

[2]Ribosomal protein L27 contributes to virulence and maduramicin resistance in Eimeria tenella. Yu Yu,Guo Huilin,Liujia Li,Guiquan Guan,Qiping Zhao,Shunhai Zhu,Jinwen Wang,Fanghe Zhao,Hui Dong,Hongyu Han. 2025

[3]Molecular characterization and analysis of a novel calcium-dependent protein kinase from Eimeria tenella. Han, H. Y.,Zhu, S. H.,Jiang, L. L.,Li, Y.,Dong, H.,Zhao, Q. P.,Kong, C. L.,Huang, B.. 2013

[4]Screening and characterization of apical membrane antigen 1 interacting proteins in Eimeria tenella. Han, Hongyu,Xue, Pu,Dong, Hui,Zhu, Shunhai,Zhao, Qiping,Huang, Bing,Huang, Bing.

[5]Molecular Characterization And Immune Protection Of An An1-Like Zinc Finger Protein Of Eimeria Tenella. Dong, Hui,Zhao, Huanzhi,Wang, Lu,Han, Hongyu,Huang, Bing,Lv, Ling,Zhao, Qiping,Zhu, Shunhai,Liu, Guiling,Li, Zhihang,Li, Zhihang,Liu, Guiling. 2020

[6]Effect of the diclazuril on Hsp90 in the second-generation merozoites of Eimeria tenella. Shen, Xiaojiong,Wang, Congcong,Li, Tao,Yu, Lijia,Zheng, Wenli,Fei, Chenzhong,Qiu, Minqi,Xue, Feiqun,Zhu, Qingxu.

[7]Establishment of Eimeria tenella (local isolate) in chicken embryos. Jiang, L.,Zhao, Q.,Zhu, S.,Han, H.,Dong, H.,Huang, B..

[8]Action of nitromezuril against Eimeria tenella with clinically anticoccidial indices and histopathology. She, Rufeng,Fei, Chenzhong,Chen, Huiya,Wang, Xiaoyang,Wang, Mi,Zhang, Keyu,Zhang, Lifang,Wang, Chunmei,Liu, Yingchun,Zheng, Wenli,Xue, Feiqun.

[9]Molecular characterization and functional analysis of subunit 7 of eukaryotic initiation factor 3 from Eimeria tenella. Han, Hongyu,Kong, Chunlin,Dong, Hui,Zhu, Shunhai,Zhao, Qiping,Zhai, Qi,Liang, Siting,Li, Sha,Yang, Shihan,Huang, Bing,Huang, Bing.

[10]Effects of diclazuril on apoptosis and mitochondrial transmembrane potential in second-generation merozoites of Eimeria tenella. Zhou, Bianhua,Xue, Feiqun,Wang, Xiaoyang,Fei, Chenzhong,Wang, Mi,Zhang, Tao,Yao, Xiujuan,He, Peiyi,Wang, Hongwei.

[11]Identification and characterization of a cathepsin-L-like peptidase in Eimeria tenella. Liu, Renqiang,Liu, Aijun,Wang, Ming,Ma, Xueting,Cai, Jianping,Zhang, Lei,Wang, Ming.

[12]Eimeria tenella rhomboid 3 has a potential role in microneme protein cleavage. Zheng, Jun,Gong, Pengtao,Li, Mingying,Zhang, Guocai,Zhang, Xichen,Li, Jianhua,Zheng, Jun,Jia, Honglin. 2014

[13]Eimeria tenella heat shock protein 70 enhances protection of recombinant microneme protein MIC2 subunit antigen vaccination against E. tenella challenge. Zhang, Lei,Ma, Liping,Liu, Renqiang,Zhang, Yunfei,Zhang, Shouping,Hu, Chunmei,Song, Meng,Wang, Ming,Cai, Jianping. 2012

[14]Eimeria tenella: Expression profiling of toll-like receptors and associated cytokines in the cecum of infected day-old and three-week old SPF chickens. Cai, Jianping,Zhang, Lei,Liu, Renqiang,Ma, Liping,Wang, Yingwei,Pan, Baoliang,Wang, Ming. 2012

[15]Eimeria tenella: Interleukin 17 contributes to host immunopathology in the gut during experimental infection. Liu, Renqiang,Song, Meng,Hu, Yanxin,Pan, Baoliang,Wang, Ming,Zhang, Lei,Wang, Ming,Cai, Jianping,Cai, Jianping. 2013

[16]Proteomic Analysis Of The Second-Generation Merozoites Of Eimeria Tenella Under Nitromezuril And Ethanamizuril Stress. Li, XY, Liu, LL, Zhang, M, Zhang, LF, Wang, XY, Wang, M, Zhang, KY, Liu, YC, Wang, CM, Xue, FQ, Fei, CZ. 2019

[17]Identification Of An Interaction Between C.alcium-Dependent Protein Kinase 4 ( Etcdpk4) And Serine Protease Inhibitor (Etserpin) In Eimeria Tenella. Lv, L, Huang, B, Zhao, QP, Zhao, ZP, Dong, H, Zhu, SH, Chen, T, Yan, M, Han, HY. 2018

[18]Anticoccidial Activity of Qinghao Powder Against Eimeria tenella in Broiler Chickens. Ling Wang,Wenzhu Guo,Shahbaz Ul Haq,Zhiting Guo,Dongan Cui,Feng Yang,Feng Cheng,Xiaojuan Wei,Jiawen Lv. 2021

[19]Molecular characterization and analysis of the ATPase ASNA1 homolog gene of Eimeria tenella in a drug sensitive strain and drug resistant strains. Yu Yu,Hui Dong,Qiping Zhao,Shunhai Zhu,Shanshan Liang,Qingjie Wang,Haixia Wang,Shuilan Yu,Bing Huang,Hongyu Han. 2021

[20]FnCas12a/crRNA-Mediated Genome Editing in Eimeria tenella. Peipei Cheng,Zhihao Zhang,Fayu Yang,Shuo Cai,Lina Wang,Chunmei Wang,Mi Wang,Yingchun Liu,Chenzhong Fei,Lifang Zhang,Feiqun Xue,Feng Gu. 2021

作者其他论文 更多>>