数字农科院2.0

Type-B response regulator RRB12 regulates nodule formation in Lotus japonicus

文献类型： 外文期刊

作者： Jingjing Cao;Yu Zhou;Tao Tian;Jie Ji;Yan Deng;Yuhao Guan;Yongmei Qi;Longxiang Wang;Longlong Wang;Yibo Huang;Qiuling Fan;Deqiang Duanmu

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关键词： （0-1-6）Cytokinin;Infection thread;Nodule inception;Nodule organogenesis;Symbiotic nitrogen fixation;Type-B response regulator

期刊名称： BMC Biology

ISSN： 1741-7007

年卷期： 2025 年 22 卷 1 期

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收录情况： SCIE（2025版）

摘要： Background: The mutualistic beneficial relationship between legume plants and rhizobia enables the growth of plants in nitrogen-limiting conditions. Rhizobia infect legumes through root hairs and trigger nodule organogenesis in the cortex. The plant hormone cytokinin plays a pivotal role in regulating both rhizobial infection and the initiation of nodule development. However, the mechanism used by the cytokinin output module to control symbiosis remains poorly documented. Results: In this study, we identified a cytokinin signaling output component encoded by the Type-B RESPONSE REGULATOR (RRB) gene, LjRRB12, which is expressed in Lotus japonicus nodule primordia and young nodules. Disruption of LjRRB12 leads to a reduction in nodulation and to an increase in the number of infection threads. Overexpression of LjRRB12D76E, an active form of the LjRRB12 protein, induces nodule-like structures in wild type and hit1 (hyperinfected 1/lotus histidine kinase 1) mutants but not in nin2 (nodule inception 2) mutants. Additionally, we utilized nCUT&Tag and EMSA to demonstrate that LjRRB12 can bind a CE (cytokinin response element) from the LjNIN promoter. Conclusions: Our results provide a deeper understanding of nodule organogenesis by establishing a link between the cytokinin signal and the transcriptional regulation of LjNIN.

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