数字农科院2.0

J亚型禽白血病病毒的拯救与鉴定

文献类型： 中文期刊

作者： 王超;缪华先;谢宝婵;张伟伟;吴润;刘光清

作者机构：

关键词： J亚群禽白血病病毒;ALV-J;感染性克隆

期刊名称：中国预防兽医学报

ISSN： 1008-0589

年卷期： 2011 年 33 卷 11 期

页码： 837-840

收录情况： 北大核心 ; CSCD

摘要： 为鉴定含J亚群禽白血病病毒(ALV-J)全长cDNA分子克隆(pBlALV)的感染性,首先将pBlALV转染CEF细胞,拯救出病毒(rALV),然后连续传代3次,对拯救病毒进行增殖培养。分别利用RT-PCR、westernblot、IFA等方法,对拯救病毒进行鉴定,并应用实时荧光定量PCR方法对拯救病毒的复制动力学进行了测定。研究结果证明,本研究成功拯救出了具有感染性的rALV-J,为研究禽白血病的致病机理和探讨新的防制措施等提供了良好的ALV的反向遗传操作技术平台。

分类号： S852.65

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