数字农科院2.0

猪带绦虫AgB与猪CD58或IFN-γ共表达质粒的构建及其在BHK-21细胞中的表达

文献类型: 中文期刊

作者: 侯俊玲;景志忠;郑亚东;胡志敏;骆学农;蒙学莲;窦永喜;刘军龙;才学鹏

作者机构:

关键词: 猪囊尾蚴;AgB;CD58;IFN-γ;转染;BHK-21细胞

期刊名称:畜牧兽医学报

ISSN: 0366-6964

年卷期: 2007 年 38 卷 07 期

页码: 694-699

收录情况: 北大核心 ; CSCD

摘要: 猪囊尾蚴病是一种重要的人畜共患病,研制有效、安全、廉价的疫苗是当前主要的研究方向之一。本试验通过PCR方法,从含猪囊尾蚴B抗原(AgB)、猪CD58和猪IFN-γ的重组克隆质粒中扩增出AgB、CD58和IFN-γ基因,然后将其克隆到真核表达载体pBudCE4.1中。重组表达质粒鉴定后,在脂质体作用下转染BHK-21细胞,通过间接免疫荧光(IFA)或直接免疫荧光试验分别检测AgB、CD58和IFN-γ在BHK-21中的表达。结果显示,AgB、CD58和IFN-γ在BHK-21细胞中都成功表达,这为研制抗猪囊尾蚴DNA疫苗奠定了坚实基础。

分类号: S858.28`S852.5

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