数字农科院2.0

Pseudomonas stutzer S12过氧化氢酶基因的表达及酶学特性研究

文献类型： 中文期刊

作者： 赵书雪;刘晓青;伍宁丰;田健

作者机构：

关键词： Pseudomonas stutzeri; 过氧化氢酶; 酶学特性; 异源表达

期刊名称：中国农业科技导报

ISSN： 1008-0864

年卷期： 2021 年 23 卷 11 期

页码：

收录情况： 北大核心（2020版） ; CSCD（2020-2021年度） ; 农林核心（2020版） ; 科技核心（2020版）

摘要： 商品过氧化氢酶目前大部分来源于动物肝脏和黑曲霉发酵,但是这些酶通常热稳定性不好、酶活力低且售价高。为了解决这一问题,从垃圾渗滤液中筛选出来一株高产过氧化氢酶的菌株Pseudomonas stutzeri S12,通过全基因组测序和基因鉴定发现了该菌株中编码过氧化氢酶的基因katE,将其在E.coli BL21（DE3）中异源表达和纯化。KatE的最适温度为35℃,最适pH为8.0,酶比活可达到1 349 U·mg-1,该酶在10～40℃范围内的酶活力较高,具有较为广泛的温度适应性。KatE具有较好的热稳定性,50℃处理5 h还剩余70%的酶活,60℃孵育2 h后剩余酶活仍有50%。其动力学性质Vmax为16 666μmol·L-1·min-1,Km为100.5 mmol·L-1。该酶在中低温条件下酶活较好,热稳定性较好,且酶活力较高,为开发新型商品过氧化氢酶提供了新的材料。

分类号：

  • 相关文献

[1]Involvement of the ammonium transporter AmtB in nitrogenase regulation and ammonium excretion in Pseudomonas stutzeri A1501. Zhang, Tao,Yan, Yongliang,He, Sheng,Ping, Shuzhen,Alam, Khandakar Mohiul,Han, Yunlei,Liu, Xiaodong,Lu, Wei,Zhang, Wei,Chen, Ming,Lin, Min,Zhang, Tao,Xiang, Wensheng,Wang, Xiangjing.

[2]Identification of the nitrogen-fixing Pseudomonas stutzeri major flagellar gene regulator FIeQ and its role in biofilm formation and root colonization. Ma Yao,Ma Yao,Zhang Qiu-lei,Yang Zhi-min,Li Yun,Yan Yong-liang,Ping Shu-zhen,Zhang Li-wen,Lin Min,Lu Wei,Yang Zhi-min.

[3]Involvement of GlnK, a P(II) protein, in control of nitrogen fixation and ammonia assimilation in Pseudomonas stutzeri A1501. He, Sheng,Chen, Ming,Xie, Zhihong,Yan, Yongliang,Li, Hongquan,Fan, Ying,Ping, Shuzhen,Lin, Min,He, Sheng,Elmerich, Claudine. 2008

[4]The novel regulatory ncRNA, NfiS, optimizes nitrogen fixation via base pairing with the nitrogenase gene nifK mRNA in Pseudomonas stutzeri A1501. Zhan, Yuhua,Yan, Yongliang,Deng, Zhiping,Chen, Ming,Lu, Wei,Lu, Chao,Shang, Liguo,Yang, Zhimin,Zhang, Wei,Wang, Wei,Li, Yun,Ke, Qi,Lu, Jiasi,Xu, Yuquan,Zhang, Liwen,Cheng, Qi,Lin, Min,Xie, Zhihong,Elmerich, Claudine.

[5]Structural and functional analysis of denitrification genes in Pseudomonas stutzeri A1501. Yan, YL,Yang, J,Chen, LH,Yang, F,Dong, J,Xue, Y,Xu, XY,Zhu, YF,Yao, ZJ,Lin, M,Wang, YP,Jin, Q.

[6]High-Frequency Targeted Mutagenesis in Pseudomonas stutzeri Using a Vector-Free Allele-Exchange Protocol. Gomaa, Ahmed E.,Deng, Zhiping,Yang, Zhimin,Shang, Liguo,Zhan, Yuhua,Lu, Wei,Lin, Min,Yan, Yongliang.

[7]Insight rifampicin-resistant (rpoB) mutation in Pseudomonas stutzeri leads to enhance the biosynthesis of secondary metabolites to survive against harsh environments. Khandakar Mohiul Alam,林敏、燕永亮,Md Ariful Islam,Ahmed Gaber,Akbar Hossain. 2022

[8]The Sigma Factor AlgU Regulates Exopolysaccharide Production and Nitrogen-Fixing Biofilm Formation by Directly Activating the Transcription of pslA in Pseudomonas stutzeri A1501. Yahui Shao,Changyan Yin,Fanyang Lv,Shanshan Jiang,Shaoyu Wu,Yueyue Han,Wei Xue,Yiyuan Ma,Juan Zheng,Yuhua Zhan,Xiubin Ke,Wei Lu,Min Lin,Liguo Shang,Yongliang Yan. 2022

[9]Effect of Pseudomonas stutzeri F2 on rearing water quality and growth, innate immunity, visceral morphology and gut microbiota structure of juvenile spotted seabass (Lateolabrax maculatus). Weilai Fu,Peifeng Duan,Qiang Wang,Junying Song,Yunshuang Wang,Zhen Zhang,Pan Wang,Huihui Jiang,Xian Zhang,Guolong Song,Zhiming Rao. 2023

[10]Integrated Hfq-interacting RNAome and transcriptomic analysis reveals complex regulatory networks of nitrogen fixation in root-associated Pseudomonas stutzeri A1501. Lv, Fanyang,Zhan, Yuhua,Feng, Haichao,Sun, Wenyue,Yin, Changyan,Han, Yueyue,Shao, Yahui,Xue, Wei,Jiang, Shanshan,Ma, Yiyuan,Hu, Haonan,Wei, Jinfeng,Yan, Yongliang,Lin, Min. 2024

作者其他论文 更多>>