数字农科院2.0

新疆一号冰川土壤细菌多样性的研究

文献类型: 中文期刊

作者: 石鹏君;孟昆;伍宁丰;付建红;姚斌;李江;邵娜

作者机构:

关键词: 冰川土壤;DGGE;细菌多样性

期刊名称:微生物学通报

ISSN: 0253-2654

年卷期: 2006 年 33 卷 04 期

页码: 58-63

收录情况: 北大核心 ; CSCD

摘要: 应用变性梯度凝胶电泳(DGGE)技术分离PCR扩增的16S rDNA来研究土壤微生物的多样性。直接从新疆一号冰川不同海拔高度的土壤样品中提取总DNA。用两套细菌通用引物分别扩增16S rDNA的V3和V6/V9高变区的特异性片段,PCR产物进行DGGE分析。PCR-DGGE图谱表明,PCR产物经DGGE检测到的条带清晰且分离效果好。结果表明,PCR-DGGE是一种快速研究微生物群落结构的有效方法。

分类号: S154.3

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