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辣椒种内高密度遗传图谱的构建

文献类型： 中文期刊

作者： 段蒙蒙;王宁;毛胜利;张正海;王立浩;张宝玺

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关键词： 辣椒;单核苷酸多态性;SLAF-seq;遗传图谱

期刊名称：中国园艺学会2014年学术年会论文摘要集

ISSN：

年卷期： 2014 年

页码： 1

收录情况： CSCD

摘要： 利用简化基因组测序技术进行大规模高通量SNP分型是当前国际上动植物基因组学研究热点。此类技术可分为3类:SLAF-seq技术、RAD技术和2b-RAD技术。本试验中对辣椒重组自交系及其亲本进行重测序,采用SLEF-seq方法挖掘多态性SNP标记,同时筛选普遍使用的多态性SSR标记,以上述两种标记建立高密度的辣椒遗传图谱,为重要性状的QTL定位及辣椒育种奠定基础。H3(Capsicumannuum)为优良辣椒自交系,抗白粉病,果实指形。83-60(C.annuum)感疫病,感白粉病,果实方灯笼形。H3×83-60RILsF7群体包含100个单株,群体记为RILs(H3×83-60)。采用SL...

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