检测与诊断猪圆环病毒PCR技术的建立及病毒的分离鉴定
文献类型: 中文期刊
作者: 崔尚金;李媛;全艳平;李建伟;胡守萍;尹训南;谷守林;童光志
作者机构:
关键词: 断奶猪多系统衰竭综合征;猪圆环病毒2型;PCR;分离鉴定
期刊名称:畜禽业
ISSN: 1008-0414
年卷期: 2006 年 01 期
页码: 14-17
摘要: 本研究建立了检测与诊断猪圆环病毒的PCR方法,并对该方法进行了标准化研究。该方法快速、敏感、特异性强,可扩增10ng的阳性质粒的DNA,可以从30μL病料组织悬液或全血中扩增检测出PCV,并且该方法可以区分PCV1和PCV-2,对其它病原微生物如PPV、PRRSV、SIV以及许多细菌不能检出。应用此方法对6省市的12个猪场的225份病料进行了检测,对阳性病料进行了病毒分离,对分离的病毒进行了电镜观察与序列测定,结果证实分离的病毒为PCV-2。
分类号: S852.65
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