数字农科院2.0

用ABC-ELISA检测羊流产衣原体抗体的研究

文献类型： 中文期刊

作者： 杨子平;张秉彝;杨学礼

作者机构：

关键词： 衣原体;ABC-ELISA;ELISA;CFT

期刊名称：中国人兽共患病杂志

ISSN： 1002-2694

年卷期： 1990 年 6 卷 02 期

页码： 38-41

摘要： 本研究以CFT和常规ELISA为基准和对照,首次建立了检测羊流产衣原体抗体的ABC-ELISA法,并对其重复性、特异性及敏感性等进行了比较。同批次和不同批次试验的可重复性良好;与其它9种羊病阳性血清均无交叉反应,试验能被特异性阻断;3种方法中,以ABC-ELISA的敏感性最高,分别为常规ELISA和CFT的1.89倍和17.54倍。羊流产衣原体绵羊源和山羊源两株抗原的交叉替代试验表明,它们具有通用性。

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