H5N1亚型禽流感病毒胶体金诊断试剂盒的初步研究
文献类型: 中文期刊
作者: 崔尚金;姜建宏;李曦;符芳;孟庆文;童光志
作者机构:
关键词: 胶体金免疫层析法(GICA);琼脂免疫扩散实验(AGID);RT-PCR
期刊名称:
ISSN:
年卷期: 2004 年
页码: 5
收录情况: CSCD
摘要: 本实验建立了一种快速的H5N1亚型禽流感病毒胶体金诊断方法。将制备好的H5N1亚型禽流感病毒多克隆抗体与但因子血清进行胶体金标记,依据胶体金免疫层析的原理构建检测试纸条。经特异性交叉试验、重复性试验、灵敏度测试后,对哈尔滨兽医研究所动物疫病诊断与流行病学中心提供的10份疑似尿囊液样品进行检测,并与RT-PCR等方法进行比较,结果与RT-PCR检测相一致。实验表明H5N1亚型禽流感病毒胶体金诊断方法具有简便、准确、灵敏度高等优点,适于流感病毒的早期快速诊断。
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