数字农科院2.0

大白菜抗TuMV分子标记辅助选择技术研究

文献类型： 中文期刊

作者： 李巧云;张志刚;赵智中;刘栓桃;王立华;高会超;李溢真;徐文玲;刘贤娴;刘辰

作者机构：

关键词： 大白菜;TuMV;抗性基因;分子标记应用

期刊名称：山东农业科学

ISSN： 1001-4942

年卷期： 2017 年 49 卷 2 期

页码： 10-14

摘要： 分别以大白菜TuMV国家级抗源材料8407和高感TuMV的核心种质材料冠291为亲本构建分离群体,为验证TuMV抗性基因TuRBCS01两侧紧密连锁的分子标记m Br4055和Br ID10723的检测准确率,对上述两个亲本F_2代分离群体的107个单株进行自交构建F_(2∶3)家系,并对每个家系的TuMV抗性进行鉴定,以判断原F_2单株的TuMV抗性及基因型,同时利用上述两个标记引物对F_2代107个单株进行检测,根据检测结果及抗病性鉴定结果计算标记检测的准确率。结果表明,两标记均检测为纯合抗病的株系有22株,其中有2株经抗病性验证为杂合抗病,其余均为纯合抗病,检测准确率为90.9%;两标记均检测为杂合抗病的有48株,经抗病性验证,其中5株为纯合抗病,5株为纯合感病,其余均为杂合抗病,检测准确率为79.2%;两标记均检测为纯合感病的有23株,经抗病性验证,其中4株为杂合抗病,1株为纯合抗病,鉴定准确率为78.3%。上述检测结果为更好地利用标记mBr4055和BrID10723进行分子标记辅助选择奠定了基础。

分类号： S436.341

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