数字农科院2.0

内生菌产抑制油菜菌核病菌生长的活性物质发酵条件研究

文献类型: 中文期刊

作者: 涂勇强;陈梦雅;余永廷;严准;孙向平

作者机构:

关键词: 油菜菌核病;;拮抗作用;;生防试验;;发酵优化;;内生细菌

期刊名称:湖南农业科学

ISSN: 1006-060X

年卷期: 2019 年 NULL 卷 12 期

页码: 6-10

摘要: 从苎麻根中获得一株对油菜菌核病菌——核盘菌具有较强抑菌作用的内生细菌G12,根据菌株形态学特征、生理生化特性, 结合 16SrDNA 分析结果对其进行鉴定,初步判断 G12 菌株为短小芽孢杆菌(Bacilluspumilus);进一步采用单因素试验分析碳源、 氮源、无机盐,以及温度、转速、pH 值、培养时间对菌株 G12 产抑菌活性物质的影响,并通过三因素三水平正交试验对发酵条 件进行了优化。结果表明 G12 菌株产抑制油菜菌核病菌的活性物质的最佳发酵条件为:玉米粉 1.5%,牛肉膏 1.5%,硫酸钙 0.2%, 发酵时间 72 h,发酵温度 37 ℃,培养液 pH 值 7,转速 200 r/min,接种量为 1%。

分类号: TS255.1`TQ920.6

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