数字农科院2.0

微波炉烘干棉花叶片用于DNA提取的效果分析

文献类型: 中文期刊

作者: 付小琼;李超;刘逢举;刘媛;彭军

作者机构:

关键词: 微波炉烘干;DNA;棉花;叶片

期刊名称:中国棉花

ISSN: 1000-632X

年卷期: 2020 年 NULL 卷 5 期

页码: 23-26

收录情况: 农林核心(2020版) ; 科技核心(2020版)

摘要: 采集棉花未展真叶、平展嫩叶和平展成熟叶用微波炉中火烘干后提取DNA,简单序列重复(Simple sequence repeat,SSR)引物扩增后的图谱显示未展真叶的DNA扩增效果不好.进一步开展了用平展子叶和全展真叶烘干的试验,发现平展子叶和全展真叶在微波炉低火、中火和高火条件下烘干后均能获得质量较好的DNA,能够满足后续分子试验的要求.烘干叶片密闭存放1周后仍能获得较好的DNA.烘干叶片提取的DNA OD260/OD280比值偏高,但不影响后续的SSR分子鉴定试验.

分类号: S562

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