数字农科院2.0

牛体内囊胚全转录组模式解析

文献类型： 中文期刊

作者： 王晶晶;张培培;郝海生;杜卫华;庞云渭;权国波;赵善江;邹惠影;朱化彬;赵学明

作者机构：

关键词： 牛体内囊胚;mRNA;lncRNA;circRNA

期刊名称：畜牧兽医学报

ISSN： 0366-6964

年卷期： 2020 年 NULL 卷 6 期

页码： 1271-1280

收录情况： 北大核心（2020版） ; CSCD（2020-2021年度） ; 农林核心（2020版） ; 科技核心（2020版）

摘要： 旨在解析牛体内囊胚的全转录组模式.本研究选择3头15月龄以上、健康状况良好的泌乳奶牛,采用超排获得体内囊胚,采用单细胞全转录组测序技术检测囊胚mRNA、lncRNA、circRNA转录模式,并采用生物信息学工具对其进行分析.试验获得牛体内囊胚约19 975个mRNAs、12 715个novel lncRNAs及887个circRNAs.mRNA有12种可变剪切方法,以转录起始区域可变剪切(TSS)为主.采用基因本体(GO)和相关信号通路(KEGG)对其分析发现,生物功能主要富集在新陈代谢、细胞膜和蛋白结合、细胞通讯、细胞组织成分、生长发育等功能,Pathway主要富集在细胞周期、膜运输、染色体组织调控等通路;circRNA有6种类型,其中CLASSIC类型circRNA数量最多,这种剪切方式会形成成环的外显子circRNA(EcircRNA).本研究阐明了牛体内囊胚的全转录组模式,为全面了解牛体内囊胚提供了新的思路.

分类号： S823

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