数字农科院2.0

RAPD标记鉴别四个水牛品种的初步研究

文献类型: 中文期刊

作者: 于农淇;郑海英;杨春艳;鄢胜飞;李孟琪;黄加祥;尚江华

作者机构:

关键词: 水牛;RAPD;品种鉴定

期刊名称:基因组学与应用生物学

ISSN: 1674-568X

年卷期: 2020 年 NULL 卷 4 期

页码: 1535-1539

摘要: 为进一步保护和开发中国本地水牛和国外引进水牛的遗传资源,建立快速区分鉴别本地和引进水牛品种的方法.设计57条随机扩增多态性(randomly amplified polymorphic DNA, RAPD)引物,对本地沼泽型水牛和国外引进的河流型(摩拉,尼里拉菲和地中海)水牛之间的遗传差异进行了调查.以128头水牛DNA样本为模板,通过PCR扩增从57条RAPD引物中筛选并优化反应条件.实验结果显示,筛选得到了5条可用于区分本地沼泽型水牛与引进河流型水牛的引物(OPG6, OPG7, S11, S40和S1013),建立了RAPD鉴别区分沼泽与河流水牛的方法.本实验的结果为中国水牛的杂交改良、良种培育和本地水牛种质资源的保护等工作提供了理论和实验依据.

分类号: S823.83

  • 相关文献

[1]利用基于RAPD标记的MCID法快速鉴定72个葡萄品种. 王玉娟,张彦,房经贵,刘崇怀,宋长年,孙欣. 2012

[2]紫花苜蓿品种鉴定的RAPD分子标记技术研究. 毛培胜,张涛,杨青川. 2007

[3]RAPD分子标记鉴定紫花苜蓿品种的反应体系优化. 张涛,杨青川,毛培胜. 2006

[4]GeneticdiversityanalysisofMagnaporthegriseafromsomeblastnurseriesofHunanprovinceusingrandomamplifiedpolymorphicDNA. NguekoRB,沈瑛,王洪凯,林福呈,徐同. 2004

[5]Random amplified polymorphism of DNA analysis in Aegilops tauschii. Kong, LR,Dong, YC,Jia, JZ. 1998

[6]Assessment of genetic diversity among selected groundnut germplasm. I: RAPD analysis. Dwivedi, SL,Gurtu, S,Chandra, S,Yuejin, W,Nigam, SN. 2001

[7]A new strategy employed for identification of sweet orange cultivars with RAPD markers. Sun, X.,Mu, Q.,Wang, C.,Wang, X. C.,Fang, J. G.,Jiang, D.. 2012

[8]RAPD study on inter-species relationships in Roegneria (Poaceae : Triticeae). Zhou, YH,Yang, JL,Zheng, YL,Yan, J,Jia, JZ,Wei, YM. 1999

[9]RAPD analysis of jasmine rice-specific genomic structure. Wu, Y. J.,Chen, Y.,Wang, J.,Zhu, C. X.,Xu, B. L.. 2006

[10]Phenotypic and genotypic changes in rapeseed after 18 years of storage and regeneration. Wu, XM,Wu, NF,Qian, XZ,Li, RG,Huang, FH,Zhu, L. 1998

[11]Morphological, cytological and molecular characterization of a novel symmetric somatic hybrid between N-tabacum and N-glauca. Sun, Y.,Xue, Q.,Zhang, X.,Ding, C.,Zhang, L.,Gong, M.,Bu, G.,Wang, W.. 2007

[12]IDENTIFICATION OF AN RAPD MARKER TIGHTLY LINKED TO BLAST RESISTANCE IN ORYZA-SATIVA L. LU, J,QIAN, HR,ZHUANG, JY,LIN, HX,ZHENG, KL. 1995

[13]Strain-typing of Lentinula edodes in China with inter simple sequence repeat markers. Zhang, Ruiying,Huang, Chenyang,Zheng, Suyue,Zhang, Jinxia,Ng, Tzi Bun,Jiang, Ruibo,Zuo, Xuemei,Wang, Hexiang.

[14]Quantitative trait loci underlying the development of seed composition in soybean (Glycine max L. Merr.). Li, Wenbin,Sun, Desheng,Du, Yuping,Chen, Qingshan,Zhang, Zhongchen,Qiu, Lijuan,Sun, Genlou.

[15]EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.. Zhang, Caiyun,Shi, Wenshuo,Ma, Keshi,Zhang, Feixiong,Li, Hongjie. 2016

[16]A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP, ISSR and RAPD Markers. Zhang Guang-qing,Qi Jian-min,Zhang Xiao-chen,Fnag Ping-ping,Tao Ai-fen,Lan Tao,Wu Wei-ren,Zhang Guang-qing,Qi Jian-min,Wu Wei-ren,Su Jian-guang,Liu Ai-min,Liu Ai-min. 2011

[17]Characteristics of molecular changes in lespedeza plants after seeds been flown on satellite. Ren Weibo,Xu Zhu,Wang Zhaolan,Deng Bo,Han JianGuo,Zhang YunWei. 2009

[18]Evidence for tetrasomic inheritance in a tetraploid Solarium commersonii (+) S-tuberosum somatic hybrid through the use of molecular markers. Barone, A,Li, J,Sebastiano, A,Cardi, T,Frusciante, L. 2002

[19]Genetic variation within Chinese and western cultivated oat accessions. Baohong, G,Zhou, X,Murphy, JP. 2003

[20]Characterization of genomes and chromosomes in partial amphiploids of the hybrid Triticum aestivum x Thinopyrum ponticum by in situ hybridization, isozyme analysis, and RAPD. Zhang, XY,Dong, YS,Wang, RRC. 1996

作者其他论文 更多>>