数字农科院2.0

玉米醇溶蛋白基因Zein启动子克隆与序列分析

文献类型： 中文期刊

作者： 崔瑞洁;许振华;谢传晓;席章营;张世煌

作者机构：

关键词： Zein;启动子;克隆

期刊名称：安徽农业大学学报

ISSN： 1672-352X

年卷期： 2010 年 37 卷 02 期

页码： 229-233

收录情况： 北大核心 ; CSCD

摘要： 启动子是细胞内控制基因转录与表达的时空特异性的重要因子。玉米醇溶蛋白基因Zein启动子控制Zein基因在胚乳中特异性表达,分离Zein启动子将为外源基因在胚乳中特异性表达等遗传操作提供基础。以玉米自交系鲁2548基因组DNA为模版,PCR扩增得到401 bp的15 kuβ-Zein启动子,PCR产物T-A克隆并测序。序列分析结果显示,作者克隆的Zein启动子序列与NCBI已报道的15 ku-Zein基因(GenBank M72708)启动子序列同源性为96%。启动子功能预测及顺式元件分析表明,所克隆的启动子序列可能具有胚乳特异性启动子功能。有关功能验证试验正在开展之中。本研究为外源基因胚乳特异性表达基因工程奠定了基础。

分类号： S513

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