数字农科院2.0

J亚群禽白血病毒gp85基因表达及初步应用

文献类型: 中文期刊

作者: 张伟伟;杨宗伟;陈宗艳;王超;李传峰;刘光清

作者机构:

关键词: J亚群禽白血病毒;囊膜糖蛋白;ELISA

期刊名称:中国动物传染病学报

ISSN: 1674-6422

年卷期: 2011 年 19 卷 01 期

页码: 17-21

摘要: J亚群禽白血病毒(Avian leukosis virus subgroup J,ALV-J)的囊膜糖蛋白GP85是亚群特异性抗原。将ALV-J的gp85基因克隆至原核表达载体pET-30a(+)EcoRⅠ和SalⅠ两个酶切位点之间,经IPTG诱导在大肠杆菌中以His-Tag融合蛋白的形式获得了高效表达,Western blot显示该表达产物具有生物学活性。GP85蛋白经纯化、复性后免疫家兔,制备了抗ALV-J GP85的多克隆抗体。以纯化的蛋白为抗原包被ELISA检测板,对疑似血清样本进行了实验室检测,检出率较高。本研究为ALV-J的诊断和流行病学调查奠定了基础。

分类号: S852.659.3

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