PCR扩增牙釉基因X和Y染色体不同序列鉴定牛早期胚胎性别
文献类型: 中文期刊
作者: 李海静;刘岩;秦彤;郝海生;张尤嘉
作者机构:
关键词: 牙釉基因;X和Y染色体;PCR;胚胎性别鉴别;牛
期刊名称:中国畜牧杂志
ISSN: 0258-7033
年卷期: 2012 年 48 卷 21 期
页码: 35-37
收录情况: 北大核心
摘要: 本实验利用牛牙釉基因特异性引物扩增牛血液、成纤维细胞和胚胎DNA,旨在优化牛早期胚胎性别鉴定的方法。结果表明:实验利用两温度PCR扩增母牛DNA样品获得1条来自X染色体458 bp产物,PCR扩增公牛DNA样品获得2条产物,其中395 bp扩增产物来自Y染色体,458 bp扩增产物来自X染色体,60头已知性别牛样品鉴定的准确率为100%。实验优化了一种两温度PCR快速鉴别奶牛及其早期胚胎性别的方法。
分类号: S823
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