数字农科院2.0

Metabolic mechanism of a novel carboxylesterase YvaK in Priestia aryabhattai DPX-1 for carbamate insecticide indoxacarb detoxification

文献类型： 外文期刊

作者： Ping Zou;Zijing Li;Shengyang Li;Yuehan Geng;Xin Ma;Xiangwei Wu;Rimao Hua;Liancheng Fang

作者机构：

关键词： Biodegradation kinetics;Carboxylesterase;Detoxification;Metabolic mechanism;Priestia aryabhattai

期刊名称： Journal of Hazardous Materials

ISSN： 0304-3894

年卷期： 2025 年 495 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Carbamates, organophosphates, pyrethroids, and other ester bond-containing insecticides are widely present in agricultural fields and aquatic environments, posing residue risks and threatening human health. Microbial degradation represents the primary metabolic pathway for these insecticides, yet it often generates highly toxic metabolites. In this study, we isolated a high-efficiency indoxacarb-degrading strain, Priestia aryabhattai DPX-1, which can metabolize 68 % of 5 mg/L indoxacarb within 24 h without producing the high-toxicity N-decarbomethoxylated metabolite (DCJW). High-resolution mass spectrometry identified a novel metabolite M513, exhibiting 1–2 orders of magnitude lower acute and chronic toxicity to aquatic organisms compared to indoxacarb. The discovery of M513 reveals a new indoxacarb metabolic pathway. Concurrently, through omics analysis, we identified a novel indoxacarb-degrading key gene yvaK in strain DPX-1, encoding a carboxylesterase. The structure of enzyme YvaK was deconstructed via the AlphaFold2 AI model. Domain analysis revealed that YvaK contains a conserved nucleophilic elbow domain composed of 91Gly-92Leu-93Ser-94Leu-95Gly and an oxyanion hole domain formed by 95Gly-96Gly. Molecular docking and site-directed mutagenesis further elucidated the catalytic mechanism. Indoxacarb could stably bind to the carboxylesterase YvaK through hydrogen bonding, further enters the catalytic center via a hydrophobic channel, and ultimately hydrolysis under nucleophile attack to generate M513. These findings provide novel and safer strategies and methodologies for the bioremediation of ester bond-containing insecticides.

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