数字农科院2.0

Polydopamine nanoparticle-mediated, click chemistry triggered, microparticle-counting immunosensor for the sensitive detection of ochratoxin A

文献类型： 外文期刊

作者： Rui Chen;Yongzhen Dong;Feng Hong;Xiya Zhang;Xiaohong Wang;Jia Wang;Yiping Chen

作者机构：

关键词： Cu(I)-catalyzed azide-alkyne cycloaddition;Ochratoxin A;Particle counting;Polydopamine nanoparticles

期刊名称： Journal of Hazardous Materials

ISSN： 0304-3894

年卷期： 2022 年 428 卷

页码：

收录情况： SCIE（2022版） ; EI（2022版）

摘要： A rapid and accurate detection method is needed for the quantitation of ochratoxin A in agricultural products due to its high toxicity. A microparticle-counting immunosensor based on polydopamine nanoparticle-mediated click chemistry was established for the highly-sensitive detection of ochratoxin A. Polydopamine nanoparticles with good biocompatibility and a strong metal-chelating ability were synthesized and conjugated with the antibody. The Coupled compounds were then used as an immune carrier to change the Cu2+ concentration via an immuno-reaction. Some of the remaining Cu2+ ions were reduced to Cu+ ions, which caused azide-polystyrene microspheres and alkyne-polystyrene microspheres to aggregate via a Cu+ ion-mediated click reaction. Particle counting was used to distinguish changes in the sizes of the polystyrene microspheres from dispersed to aggregated to detect ochratoxin A. It showed a wide linear detection range of 0.5–800 ng/mL, and a detection limit of 0.2 ng/mL. This assay provides an attractive analytical tool for the accurate detection of trace targets in complex samples.

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