数字农科院2.0

Sequencing of the cDNA variable region of a lumpy skin disease virus ORF123 monoclonal antibody and its cross-neutralizing activity analyses against Capripoxvirus members

文献类型： 外文期刊

作者： Fangping Wang;Shasha Wang;Lina Tong;Huibao Wang;Haotai Chen;Xiangwei Wang;Xiangping Yin;Yuefeng Sun;Xiaolong Gao;Shanhui Ren

作者机构：

关键词： Cross-reactivity and neutralizing activity;Goat poxvirus;Lumpy skin disease virus;Monoclonal antibody sequencing;ORF123;Sheep poxvirus

期刊名称： Virology

ISSN： 0042-6822

年卷期： 2025 年 613 卷

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收录情况： SCIE（2025版）

摘要： Monoclonal antibody sequencing is crucial for enhancing our understanding of the relationship between antibody neutralization and broad-spectrum binding. Our previous study systematically described a B-cell monoclonal antibody (mAb) derived from lumpy skin disease virus (LSDV) ORF123, which exhibits cross-reactivity with goatpoxvirus (GTPV) and sheeppoxvirus (SPPV). Here, the LSDV ORF123 mAb was sequenced for the first time using the HybSeq HT™ next-generation sequencing technique. Sequencing analyses showed that the variable heavy (VH) and light (VL) chains of LSDV ORF123 mAb had significant sequence similarity. Subsequently, the top 1 VH and VL of this LSDV ORF123 mAb were synthesized and expressed. Western blotting analyses further identified that the complementary determinant regions (CDR)1, CDR2, and CDR3 are indispensable for antigen-antibody recognition. Crucially, the co-expression of VH + VL of this LSDV ORF123 mAb retained its cross-neutralizing activity against LSDV, GTPV, and SPPV. Variable region sequencing of monoclonal antibodies provides a reference for improving the specificity and affinity of potential therapeutic antibody design. The cross-binding and neutralizing activity of the co-expression of VH + VL of the LSDV ORF123 mAb strengthens our understanding of the antigenic similarity among different members of the genus Capripoxviruses.

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