Development of a competitive ELISA using monoclonal antibodies for detecting neutralizing antibodies against Peste-des-petits-ruminants virus
文献类型: 外文期刊
作者: Linjie Hu;Haoyue Xu;Xueliang Zhu;Hongjiao Peng;Ruixue Deng;Yuefeng Sun;Xuelian Meng
作者机构:
关键词: Competitive ELISA;Haemagglutinin protein;Monoclonal antibody;Peste-des-petits-ruminants virus
期刊名称: Veterinary Journal
ISSN: 1090-0233
年卷期: 2025 年 313 卷
页码:
收录情况: SCIE(2025版)
摘要: Peste-des-petits-ruminants (PPR) is an acute, contact infectious disease caused by peste-des-petits-ruminants virus (PPRV) infection. PPRV mainly infects goats, sheep and other small ruminants, posing a serious threat to the development of the livestock industry and the safety of wildlife. In this study, a competitive ELISA (c-ELISA) method for detecting neutralizing antibodies against PPRV was developed using the extracellular domain of haemagglutinin (tH) protein and a monoclonal antibody against tH protein. The results showed that the specificity and sensitivity of c-ELISA were 99.38% and 100%, respectively. The agreement rate of the c-ELISA and viral neutralization test (VNT) was 96.65%, suggesting that the c-ELISA could detect neutralizing antibody against PPRV and could be used for protective immunity evaluation.
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