数字农科院2.0

Targeting of the cellular ATM rather than ATR kinase exhibits therapeutic potential during the lumpy skin disease virus infection in vivo and in vitro

文献类型： 外文期刊

作者： Shanhui Ren;Haotai Chen;Shasha Wang;Zaib Ur Rehman;Xiaolong Gao;Xue Yang;Xiangwei Wang;Xiangping Yin;Jianlin Han;Yuefeng Sun

作者机构：

关键词： ATM kinase;ATR kinase;DNA damage response;Lumpy skin disease virus;Signaling transduction

期刊名称： Antiviral Research

ISSN： 0166-3542

年卷期： 2025 年 243 卷

页码：

收录情况： SCIE（2025版）

摘要： Due to the lack of timely vaccine prevention and effective drug treatment, lumpy skin disease is increasingly becoming a global epidemic, including in China. There is an urgent need to explore the pathogenic mechanism of lumpy skin disease virus (LSDV) and develop practical therapeutic approaches. The present study provides concrete evidence for the simultaneous induction and activation of nuclear ATM-mediated double-strand break and ATR kinase-dependent single-strand break signaling cascades during LSDV replication in the cytoplasm. Specific drug-inhibitory experiments targeting ATM and ATR kinase activity have showed that LSDV activates the host deoxyribonucleic acid (DNA) damage response (DDR) to facilitate viral replication via ATM-Chk2 rather than the ATR-Chk1 signaling axis in vitro. Meanwhile, animal experiments corroborated the efficacy of an inhibitor drug targeting ATM kinase in decreasing the clinical symptoms of LSDV-infected cattle in vivo. These findings highlight how LSDV exploits the nuclear DDR pathway to enhance replication in the cytoplasmic viral factory, deepening our understanding of virus-host interactions and providing a new target for developing specific antiviral drugs and interventions.

分类号：

  • 相关文献

[1]Protein kinase ATR inhibits E3 ubiquitin ligase CRL4PRL1 to stabilize ribonucleotide reductase in response to replication stress. Weiyi Bao,Weijia Zhang,Yongchi Huang,Yan Zhao,Cong Wu,Leilei Duan,Lili Wang,Shunping Yan. 2023

[2]Understanding the research advances on lumpy skin disease: A comprehensive literature review of experimental evidence. Zhengji Liang,Kaishen Yao,Shasha Wang,Juanbin Yin,Xiaoqin Ma,Xiangping Yin,Xiangwei Wang,Yuefeng Sun. 2022

[3]The comparative study revealed that the hTERT-CSF cell line was the most susceptible cell to the Lumpy skin disease virus infection among eleven cells. Chunling Ma,Shanhui Ren,Tadele Berihun Afera,Xue Yang,Yugang Lin,Xiaohong Gao,Fangping Wang,Xusheng Qiu,Xiangwei Wang,Xiangping Yin,Yuefeng Sun,Xuerui Wan,Hao tai Chen. 2023

[4]Identification of the murine osteoblastic cell MC3T3-E1 as a permissive cell line in response to lumpy skin disease virus. Ting You,Meng Wang,Hongqiang Zhang,Xiangwei Wang,Xiaolong Gao,Xiangping Yin,Yuefeng Sun,Guirong Wang,Hao tai Chen,Shanhui Ren. 2024

[5]Platform establishment of the Cre-loxP recombination system for genetic manipulation of the Lumpy skin disease virus. Shanhui Ren,Yuzhe Zhang,Xiaolong Gao,Xiangwei Wang,Lina Tong,Shasha Wang,Yuefeng Sun,Xiangping Yin,Haotai Chen. 2024

[6]Phylogenetic and pathogenic characterization of lumpy skin disease virus circulating in China. Shanhui Ren,Haotai Chen,Lvfeng Yuan,Xue Yang,Tadele Berihun Afera,Zaib Ur Rehman,Huibao Wang,Xiangwei Wang,Chunling Ma,Yuguang Lin,Xusheng Qiu,Xiangping Yin,Yuefeng Sun. 2023

[7]Lumpy Skin Disease Virus Infection Activates Autophagy and Endoplasmic Reticulum Stress-Related Cell Apoptosis in Primary Bovine Embryonic Fibroblast Cells. Jinlong Tan,Yinju Liu,Weike Li,Yongzhi Zhang,Guohua Chen,Yongxiang Fang,Xiaobing He,Zhizhong Jing. 2023

[8]Lumpy skin disease virus LSDV001 protein positively regulates inflammatory response by promoting assembly of the TAK1-TAB2/3 complex. Yang, Yu-Lin,Jia, Huai-Jie,Sun, Meng-Yao,Guo, Ai-Min,Xia, Tian,Shu, Hong-Bing,Cao, Li-Bo. 2025

[9]Lumpy Skin Disease Virus ORF137 Protein Inhibits Type I Interferon Production by Interacting with and Decreasing the Phosphorylation of IRF3. Qunhua Ke,Kaishen Yao,Min Qu,Zhengji Liang,Miaomiao Li,Xiangwei Wang,Xiangping Yin,Yuefeng Sun. 2025

[10]Lumpy skin disease virus LSDV087 positively regulates innate immune response by promoting oligomerization of MITA/STING. Li, Zhen-Zhen,Yang, Yu-Lin,Sun, Meng-Yao,Shu, Hong-Bing,Cao, Li-Bo. 2025

[11]Construction and biological characteristics analysis of a gene-deleted recombinant virus strain of LSDV-ORF151. Jiaqi Li,Qunhua Ke,Weitao Huang,Shanhui Ren,Miaomiao Li,Kaishen Yao,Xiaoqin Ma,Yuefeng Sun,Xiangping Yin. 2025

[12]Sequencing of the cDNA variable region of a lumpy skin disease virus ORF123 monoclonal antibody and its cross-neutralizing activity analyses against Capripoxvirus members. Fangping Wang,Shasha Wang,Lina Tong,Huibao Wang,Haotai Chen,Xiangwei Wang,Xiangping Yin,Yuefeng Sun,Xiaolong Gao,Shanhui Ren. 2025

[13]Replication and Virulence in Cattle of the First Lumpy Skin Disease Virus Isolated in Xinjiang, China (2019). Zhang, Minmin,Li, Shaohan,Shi, Yujie,Xu, Xiaolong,Wen, Zhiyuan,Ge, Jinying,Zhang, Qiwei,Lu, Xinyin,Yin, Xin,Bu, Zhigao. 2025

作者其他论文 更多>>