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CsMIKC1 regulates inflorescence development and grain production in Cannabis sativa plants

文献类型： 外文期刊

作者： Gencheng Xu;Yongbei Liu;Shuhao Yu;Dejing Kong;Kailei Tang;Zhigang Dai;Jian Sun;Chaohua Cheng;Canhui Deng;Zemao Yang;Qing Tang;Chao Li;Jianguang Su;Xiaoyu Zhang

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期刊名称： Horticulture Research

ISSN： 2662-6810

年卷期： 2024 年 11 卷 8 期

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收录情况： SCIE（2024版） ; ; CSCD（2023-2024年度） ; ; 科技核心（2024版） ; ; 农林核心（2020版）

摘要： Female inf lorescence is the primary output of medical Cannabis. It contains hundreds of cannabinoids that accumulate in the glandular trichomes. However, little is known about the genetic mechanisms governing Cannabis inf lorescence development. In this study, we reported the map-based cloning of a gene determining the number of inf lorescences per branch. We named this gene CsMIKC1 since it encodes a transcription factor that belongs to the MIKC-type MADS subfamily. Constitutive overexpression of CsMIKC1 increases inf lorescence number per branch, thereby promoting f lower production as well as grain yield in transgenic Cannabis plants. We further identified a plant-specific transcription factor, CsBPC2, promoting the expression of CsMIKC1. CsBPC2 mutants and CsMIKC1 mutants were successfully created using the CRISPR-Cas9 system; they exhibited similar inf lorescence degeneration and grain reduction. We also validated the interaction of CsMIKC1 with CsVIP3, which suppressed expression of four inf lorescence development-related genes in Cannabis. Our findings establish important roles for CsMIKC1 in Cannabis, which could represent a previously unrecognized mechanism of inf lorescence development regulated by ethylene.

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