数字农科院2.0

Key miRNAs of chicken seminal plasma extracellular vesicles related with sperm motility regulation

文献类型： 外文期刊

作者： Xintong Han;Yunlei Li;Yunhe Zong;Yi Zhao;Lijun Jiang;Aixin Ni;Hanhan Yang;Jingwei Yuan;Hui Ma;Lin Ma;Jilan Chen;Tenghe Ma;Yanyan Sun

作者机构：

关键词： Chicken;Extracellular vesicles;miRNA;Seminal plasma

期刊名称： International Journal of Biological Macromolecules

ISSN： 0141-8130

年卷期： 2024 年 277 卷

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： MicroRNAs (miRNAs) are bio-active elements cargoed by seminal plasma extracellular vesicles extracellular vesicles (SPEVs) which are crucial for sperm function and fertility modulation. This study aimed to isolate, characterize, and identify the miRNA expression profiles in the SPEVs from high (HSM) and low sperm motility (LSM) groups that could serve as fertility biomarkers and explain the underlying mechanisms. The isolated SPEVs were round spherical structures of approximately 50–200 nm in diameter expressing molecular markers. A total of 1006 and 1084 miRNAs were detected in HSM and LSM, respectively, with 34 being differentially expressed. Their targeted genes involved in SNARE interactions in vesicular transport, Metabolic pathways, and Apelin signaling pathway, etc. The joint analysis with mRNAs of sperm and sperm storage tubules cells highlighted the cellular communication mediated by SPEVs miRNAs, where they may rule fertility by affecting sperm maturation and amino acid metabolism. SPEVs as additives could improve fertility of fresh and frozen sperm, while the knockdown of one of the differentially expressed miRNAs, miR-24-3p, diminished this effect, indicating its crucial roles. This study expands our understanding of SPEVs miRNAs mediated sperm maturation and fertility modulation, and may help to develop new therapeutic strategies for infertility and sperm storage.

分类号：

  • 相关文献

[1]Extracellular vesicle-coupled miRNA profiles of chicken seminal plasma and their potential interaction with recipient cells. Xintong Han,Yunlei Li,Yunhe Zong,Dongli Li,Jingwei Yuan,Hanhan Yang,Hui Ma,Aixin Ni,Yuanmei Wang,Jinmeng Zhao,Jilan Chen,Tenghe Ma,Yanyan Sun. 2023

[2]Correlation Between MicroRNA by Extracellular Vesicle Mediated and Antiviral Effects of Interferon Omega in Feline Peripheral Blood. Mingxing Yang,Guowei Xu,Jingyan Zhang,Zhiting Guo,Chao Liang,Yajun Li,Lei Wang,Yuxia Zhou,Yi Ru,Jianxi Li,Xuezhi Wang,Yan Sun. 2024

[3]Exploring extracellular vesicles in zoonotic helminth biology: implications for diagnosis, therapeutic and delivery. Abdul Qadeer,Abdul Wajid,Hafiz Abdul Rafey,Saqib Nawaz,Sawar Khan,Sajid Ur Rahman,Khalid J. Alzahrani,Muhammad Zahoor Khan,Mohammad Nafi Solaiman Alsabi,Hanif Ullah,Sher Zaman Safi,Zanxian Xia,Muhammad Zahoor. 2024

[4]CircGRB14 Inhibits Proliferation and Promotes Apoptosis of Granulosa Cells in Chicken Follicle Selection Through Sponging miR-12264-3p and miR-6660-3p. Huanqi Yang,Mengxiao Li,Beibei Zhang,Jinming Zhang,Yuxiang Shi,Tenghe Ma,Yanyan Sun. 2025

[5]噬菌体展示技术分析鸡贫血病毒VP1蛋白抗原结构特征. 王晓艳,高玉龙,高宏雷,付朝阳,邓小芸,林欢,祁小乐,王笑梅. 2007

[6]GlobalgeneexpressionprofileofchickenspleentissueatalaterstageofIBVinfectionrevealspathogenesisandimmunemechanisms. FengCong,ZongxiHan,FengjieWang,YuhaoShao,XiangangKong,ShengwangLiu. 2014

[7]EIAV基因转移载体启动子的比较. 韩凌霞,李亚明,曲连东,司昌德,姜骞,刘家森. 2006

[8]Research note: Chicken seminal plasma metabolome and fertility-enhancing effects of L-carnitine supplementation in semen. Yunlei Li,Lijun Jiang,Yunhe Zong,Aixin Ni,Xintong Han,Adamu Mani Isa,Jingwei Yuan,Jilan Chen,Yanyan Sun. 2025

[9]Roles Of Micrornas In T C.ell Immunity: Implications For S trategy Development Against Infectious Diseases. Giri, BR, Mahato, RI, Cheng, GF. 2019

[10]Extracellular vesicles from Fasciola gigantica induce cellular response to stress of host cells. Guo A.,Wang L.,Meng X.,Zhang S.,Sheng Z.,Luo X.,Huang W.,Wang S.,Cai X.. 2021

[11]Uterine macrophages as treatment targets for therapy of premature rupture of membranes by modified ADSC-EVs through a circRNA/miRNA/NF-κB pathway. Yuhua Gao,Ningning Mi,Ying Zhang,Xiangchen Li,Weijun Guan,Chunyu Bai. 2022

[12]Comparative Analysis Of Different Extracellular Vesicles Secreted By Echinococcus Granulosus Protoscoleces. Wu, JE, Cai, MT, Yang, J, Li, YT, Ding, JT, Kandil, OM, Kutyrev, I, Ayaz, M, Zheng, YD. 2021

[13]miR-212/132-Enriched Extracellular Vesicles Promote Differentiation of Induced Pluripotent Stem Cells Into Pancreatic Beta Cells. Chunyu Bai,Qiwei Ren,Haifeng Liu,Xiangchen Li,Weijun Guan,Yuhua Gao. 2021

[14]Extracellular vesicles derived from umbilical cord mesenchymal stromal cells alleviate pulmonary fibrosis by means of transforming growth factor-β signaling inhibition. Shi Liyan,Ren Jing,Li Jiping,Wang Dongxu,Wang Yusu,Qin Tao,Li Xiuying,Zhang Guokun,Li Chunyi,Wang Yimin. 2021

[15]Eimeria falciformis secretes extracellular vesicles to modulate proinflammatory response during interaction with mouse intestinal epithelial cells. Joshua Seun Olajide,Ling Xiong,Shunli Yang,Zigang Qu,Xiao Xu,Bin Yang,Jing Wang,Baohong Liu,Xueting Ma,Jianping Cai. 2022

[16]Comparative Proteomic Analysis of Milk-Derived Extracellular Vesicles from Dairy Cows with Clinical and Subclinical Mastitis. Mengling Wang,Meng Cai,Xiaoyan Zhu,Xuemei Nan,Benhai Xiong,Liang Yang. 2023

[17]Proteomic analysis of extracellular vesicles from tick hemolymph and uptake of extracellular vesicles by salivary glands and ovary cells. Zhengmao Xu,Yanan Wang,Meng Sun,Yongzhi Zhou,Jie Cao,Houshuang Zhang,Xuenan Xuan,Jinlin Zhou. 2023

[18]Quantitative proteomic analysis of local and systemic extracellular vesicles during Eimeria falciformis infectious cycle in the host. Joshua Seun Olajide,Zigang Qu,Shunli Yang,Bin Yang,Xiao Xu,Jing Wang,Jianping Cai. 2023

[19]Distribution and Ultrastructural Features of Telocytes in the Pars Distalis of the Rat Pituitary Gland. Chunhua, Liang,Xuebing, Bai,Yonghong, Shi,Min, Yang,Haixiang, Huang,Jianming, Yue,Zhenwei, Zhang,Qiusheng, Chen. 2023

[20]Transfer of inflammatory mitochondria via extracellular vesicles from M1 macrophages induces ferroptosis of pancreatic beta cells in acute pancreatitis. Yuhua Gao,Ningning Mi,Wenxiang Wu,Yuxuan Zhao,Fangzhou Fan,Wangwei Liao,Yongliang Ming,Weijun Guan,Chunyu Bai. 2024

作者其他论文 更多>>