数字农科院2.0

Long-24-h ahemeral light cycle improved eggshell quality of hens in late laying period

文献类型： 外文期刊

作者： Xuelu Liu;Lei Shi;Bofei Su;Aoyang Liu;Dehe Wang;Yifan Chen;Erying Hao;Hao Bai;Yanyan Sun;Yunlei Li;Jilan Chen;Hui Chen

作者机构：

关键词： Chicken;Eggshell quality;Late laying period;Light cycle

期刊名称： Poultry Science

ISSN： 0032-5791

年卷期： 2025 年 104 卷 4 期

页码：

收录情况： SCIE（2025版）

摘要： Previous study indicated that long-24-h ahemeral light cycle could ameliorate the deterioration of eggshell quality, a significant issue in the later stages of egg production; nevertheless, the underlying process remains unclear. This study explored the mechanism of the long-24-h ahemeral light cycle improving eggshell quality of hens in late laying stage. Two sets of 260 Hy-Line brown laying hens, aged 74 weeks, were randomized, with 65 chickens in each group. One was treated to a standard light cycle (24 h, 16L:8D), whereas the other was subjected to a long-24 h ahemeral light cycle (28 h, 16L:12D). The experimental cycle was 12 weeks. The results indicated that the long-24 h ahemeral light cycle did not influence laying performance (P > 0.05); however, eggshell strength and thickness was dramatically enhanced (P < 0.05). The serum levels of 1,25(OH)2D3 and ALP exhibited comparable changes, whereas PTH, PCT, and OT displayed contrasting variations between the two groups. Transcriptome sequencing revealed 889 common genes exhibiting rhythmic expression in both groups, with an average phase advance of 7.41 h. In comparison to the 16L:8D group, 97.41 % of genes exhibited phase advancement in the 16L:12D group. Based on the findings of the functional analysis, we identified four genes associated with bone metabolism and examined their expression patterns: CTNNB1, BMPR2, BMP7, and PDIA3. The expression trend of BMPR2 gene was similar to that of serum 1,25(OH)2D3, and the expression trend of BMP7 gene was similar to that of serum OT. The mRNA expression patterns of PDIA3 and BMP7 were inversely related, whereas CTNNB1 and BMPR2 exhibited comparable trends. The mRNA expression of CTNNB1, PDIA3, and BMP7 correlated with eggshell formation. The mRNA expression of BMPR2 significantly elevated during eggshell formation in the 16L:8D group, whereas it remained low during the daylight in comparison to darkness in the 16L:12D group. In summary, the change of light cycle affects the biological rhythm of laying hens, and we found four genes related to bone metabolism, CTNNB1, BMPR2, BMP7 and PDIA3, which specifically responded to the long-24 h ahemeral light cycle and possibly participated in the improvement of eggshell quality.

分类号：

  • 相关文献

[1]Effects of 28 h ahemeral light cycle on production performance, egg quality, blood parameters, and uterine characteristics of hens during the late laying period. Xuelu Liu,Lei Shi,Erying Hao,Xiangyu Chen,Ziwen Liu,Yifan Chen,Dehe Wang,Chenxuan Huang,Jiawei Ai,Min Wu,Yanyan Sun,Yunlei Li,Lijun Xu,Erdong Sun,Jilan Chen,Hui Chen. 2024

[2]噬菌体展示技术分析鸡贫血病毒VP1蛋白抗原结构特征. 王晓艳,高玉龙,高宏雷,付朝阳,邓小芸,林欢,祁小乐,王笑梅. 2007

[3]GlobalgeneexpressionprofileofchickenspleentissueatalaterstageofIBVinfectionrevealspathogenesisandimmunemechanisms. FengCong,ZongxiHan,FengjieWang,YuhaoShao,XiangangKong,ShengwangLiu. 2014

[4]EIAV基因转移载体启动子的比较. 韩凌霞,李亚明,曲连东,司昌德,姜骞,刘家森. 2006

[5]In Ovo Feeding of Arginine, Leucine, and Methionine in Broiler Breeders’ Eggs During Summer: Effects on Hatchability and Chick Oxidation, Inflammation, and Apoptosis. Huan Ge,Zhenwu Huang,Jinghai Feng,Minhong Zhang. 2025

[6]Manganese supplementation enhances the synthesis of glycosaminoglycan in eggshell membrane: A strategy to improve eggshell quality in laying hens. Xiao, J. F.,Zhang, Y. N.,Wu, S. G.,Zhang, H. J.,Yue, H. Y.,Qi, G. H.. 2014

[7]Comparative proteomics of matrix fractions between pimpled and normal chicken eggshells. Liu, Zhangguo,Song, Lingzi,Zhang, Xianfu,Lu, Lizhi,Zhang, Fuming,Linhardt, Robert J.,Zhang, Fuming,Linhardt, Robert J.,Zhang, Fuming,Linhardt, Robert J.,Zhang, Fuming,Linhardt, Robert J.,Wang, Kehua.

[8]Effect of dietary supplementation of organic or inorganic zinc on carbonic anhydrase activity in eggshell formation and quality of aged laying hens. Zhang, Y. N.,Zhang, H. J.,Wang, J.,Yue, H. Y.,Qi, X. L.,Wu, S. G.,Qi, G. H..

[9]Effect of dietary supplementation of organic or inorganic manganese on eggshell quality, ultrastructure, and components in laying hens. Zhang, Y. N.,Wang, J.,Zhang, H. J.,Wu, S. G.,Qi, G. H..

[10]Bioefficacy comparison of organic manganese with inorganic manganese for eggshell quality in Hy-Line Brown laying hens. Xiao, J. F.,Wu, S. G.,Zhang, H. J.,Yue, H. Y.,Wang, J.,Ji, F.,Qi, G. H..

[11]The partial replacement of sodium chloride with sodium bicarbonate or sodium sulfate in laying hen diets improved laying performance, and eggshell quality and ultrastructure. Yu Fu,Jing Wang,Hai-jun Zhang,Shu-geng Wu,Jian-min Zhou,Guang-hai Qi. 2021

[12]Effects of rearing systems on the eggshell quality, bone parameters and expression of genes related to bone remodeling in aged laying hens. Fu, Yu,Wang, Jing,Schroyen, Martine,Chen, Gang,Zhang, Hai-jun,Wu, Shu-geng,Li, Bao-ming,Qi, Guang-hai. 2022

[13]Effects of Selenium Yeast on Egg Quality, Plasma Antioxidants, Selenium Deposition and Eggshell Formation in Aged Laying Hens. Zhexi Liu,Yutao Cao,Yue Ai,Gang Lin,Xiaonan Yin,Linli Wang,Mengyao Wang,Bingkun Zhang,Keliang Wu,Yuming Guo,Hongbing Han. 2023

[14]TMT-based quantitative proteomic analysis reveals eggshell matrix protein changes correlated with eggshell quality in Jing Tint 6 laying hens of different ages. Zhao D.-R.,Gao L.-B.,Gong F.,Feng J.,Zhang H.-J.,Wu S.-G.,Wang J.,Min Y.-N.. 2024

[15]Decreased eggshell strength caused by impairment of uterine calcium transport coincide with higher bone minerals and quality in aged laying hens. Yu Fu,Jianmin Zhou,Martine Schroyen,Haijun Zhang,Shugeng Wu,Guanghai Qi,Jing Wang. 2024

[16]Dietary supplementation with calcitriol or quercetin improved eggshell and bone quality by modulating calcium metabolism. Yu Fu,Jianmin Zhou,Martine Schroyen,Jing Lin,Haijun Zhang,Shugeng Wu,Guanghai Qi,Jing Wang. 2024

[17]Dietary sodium sulfate supplementation improves eggshell quality, uterine ion transportation and glycosaminoglycan synthesis in laying hens. Fu, Kai-bo,Dai, Dong,Zhou, Jian-min,Zhang, Hai-jun,Wu, Shu-geng,Qi, Guang-hai,Wang, Jing. 2025

[18]TMT-Based quantitative proteomic analysis reveals age-related changes in eggshell matrix proteins and their correlation with eggshell quality in Xinyang blue-shelled laying hens. Fu, Yu,Zhao, Dan-rong,Gao, Li-bing,Zhang, Hai-jun,Feng, Jia,Min, Yu-na,Qi, Guang-hai,Wang, Jing. 2025

[19]The characterization of uterine calcium transport and metabolism during eggshell calcification of hens laying high or low breaking strength eggshell. Ying ying Yang,Dong Dai,Hai jun Zhang,Shu geng Wu,Guang hai Qi,Jing Wang. 2025

[20]Proteomic changes in extracellular vesicle and uterine fluid proteins correlated with eggshell quality in aged laying hens. Li bing Gao,Yu Fu,Yan ting Guo,Hai jun Zhang,Guang hai Qi,Jing Wang. 2025

作者其他论文 更多>>