数字农科院2.0

A cluster of mutagenesis revealed an osmotic regulatory role of the OsPIP1 genes in enhancing rice salt tolerance

文献类型： 外文期刊

作者： Tao, Leyuan;Wang, Bing;Xin, Shichao;Li, Wei;Huang, Shengcai;Liu, Laihua;Cui, Jing;Zhang, Qianru;Cheng, Xianguo

作者机构：

关键词： Aquaporin;Multiplex gene editing;CRISPR/Cas9;OsPIP1 genes;Salt tolerance;Rice(Oryza sativa L.)

期刊名称： CROP JOURNAL

ISSN： 2095-5421

年卷期： 2023 年 11 卷 4 期

页码：

收录情况： SCIE（2023版） ; ; CSCD（2023-2024年度） ; ; 科技核心（2023版） ; ; 农林核心（2020版）

摘要： Aquaporins play important regulatory roles in improving plant abiotic stress tolerance. To better understand whether the OsPIP1 genes collectively dominate the osmotic regulation in rice under salt stress, a cluster editing of the OsPIP1;1, OsPIP1;2 and OsPIP1;3 genes in rice was performed by CRISPR/Cas9 system. Sequencing showed that two mutants with Cas9-free, line 14 and line 18 were successfully edited. Briefly, line 14 deleted a single C base in both the OsPIP1;1 and OsPIP1;3 genes, and inserted a single T base in the OsPIP1;2 gene, respectively. While line 18 demonstrated an insertion of a single A base in the OsPIP1;1 gene and a single T base in both the OsPIP1;2 and OsPIP1;3 genes, respectively. Multiplex editing of the OsPIP1 genes significantly inhibited photosynthetic rate and accumulation of compatible metabolites, but increased MDA contents and osmotic potentials in the mutants, thus delaying rice growth under salt stress. Functional loss of the OsPIP1 genes obviously suppressed the expressions of the OsPIP1, OsSOS1, OsCIPK24 and OsCBL4 genes, and increased the influxes of Na' and effluxes of K'/H' in the roots, thus accumulating more Na' in rice mutants under salt stress. This study suggests that the OsPIP1 genes are essential modulators collectively contributing to the enhancement of rice salt stress tolerance, and multiplex editing of the OsPIP1 genes provides insight into the osmotic regulation of the PIP genes. (c) 2023 Crop Science Society of China and Institute of Crop Science, CAAS. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY license (http:// creativecommons.org/licenses/by/4.0/).

分类号：

  • 相关文献

[1]Exogenous abscisic acid represses rice flowering via SAPK8-ABF1- Ehd1 / Ehd2 pathway. Tang, Liqun,Li, Guanghao,Wang, Huimei,Zhao, Juan,Li, Zhiyong,Liu, Xixi,Shu, Yazhou,Liu, Wanning,Wang, Shuang,Huang, Jie,Ying, Jiezheng,Tong, Xiaohong,Yuan, Wenya,Wei, Xiangjin,Tang, Shaoqing,Wang, Yifeng,Bu, Qingyun,Zhang, Jian. 2024

[2]Exploiting the efficient Exo:Cas12i3-5M fusions for robust single and multiplex gene editing in rice. Wang, Wenxue,Li, Shaoya,Yang, Jiaying,Li, Jingying,Yan, Lei,Zhang, Chen,He, Yubing,Xia, Lanqin. 2025

[3]Roles Of Soybean Plasma Membrane I.ntrinsic Protein Gmpip2;9 In D rought Tolerance And Seed Development. Liu, Ruifang,Shou, Huixia,Lu, Linghong,Wang, Chuang,Lu, Linghong,Dong, Changhe,Zhou, Bin,Wang, Chuang. 2018

[4]Structural and evolutionary divergence of aquaporins in parasites. Ni, Zi-Xin,Cui, Jian-Min,Zhang, Nian-Zhang,Fu, Bao-Quan,Fu, Bao-Quan.

[5]Cloning, identification, and bioinformatics analysis of a putative aquaporin TsAQP from Trichinella spiralis. Cui, J. M.,Zhang, N. Z.,Li, W. H.,Yan, H. B.,Fu, B. Q.,Fu, B. Q.. 2015

[6]An aquaporin protein is associated with drought stress tolerance. Li, Jun,Wen, Hongyu,Wang, Zan,Gao, Hongwen,Wang, Xuemin,Li, Jun,Ban, Liping,Dzyubenko, Nikolay,Chapurin, Vladimir.

[7]TsMIP6 enhances the tolerance of transgenic rice to salt stress and interacts with target proteins. Sun, Linlin,Han, Xiaori,Zhang, Shuhui,Sun, Linlin,Yu, Guohong,Xin, Shichao,Qiang, Xiaojing,Jiang, Linlin,Zhang, Shuhui,Cheng, Xianguo,Xin, Shichao.

[8]Expression of tomato SlTIP2;2 enhances the tolerance to salt stress in the transgenic Arabidopsis and interacts with target proteins. Xin, Shichao,Yu, Guohong,Qiang, Xiaojing,Xu, Na,Cheng, Xianguo,Sun, Linlin.

[9]Identification of Plasma Membrane Aquaporin in Guard Cells of Vicia faba and Its Role in Stomatal Movement. Huang, RF,Zhu, MJ,Kang, Y,Chen, J,Wang, XC.

[10]Molecular cloning and expression analysis of tea plant aquaporin (AQP) gene family. Yue, Chuan,Cao, Hongli,Wang, Lu,Zhou, Yanhua,Hao, Xinyuan,Zeng, Jianming,Wang, Xinchao,Yang, Yajun,Wang, Lu,Zeng, Jianming,Wang, Xinchao,Yang, Yajun,Yue, Chuan,Cao, Hongli,Wang, Lu,Zhou, Yanhua,Hao, Xinyuan,Zeng, Jianming,Wang, Xinchao,Yang, Yajun.

[11]Structural And Evolutionary Divergence Of A.quaporins In Parasites. Ni, ZX, Cui, JM, Zhang, NZ, Fu, BQ. 2017

[12]A Novel Aquaporin 12-Like Protein F.rom Chilo Suppressalis: Characterization A nd Functional Analysis. Song, Jie,Song, Jie,Wang, Guirong,Xu, Jing,Xu, Jing,Lu, Ming-Xing,Du, Yu-Zhou,Du, Yu-Zhou,Du, Yu-Zhou,Lu, Ming-Xing,Liu, Yang. 2019

[13]Two Aquaporin Genes, GhPIP2;7 and GhTIP2;1, Positively Regulate the Tolerance of Upland Cotton to Salt and Osmotic Stresses. Anhui Guo,Jianfeng Hao,Ying Su,Bin Li,Nan Zhao,Meng Zhu,Yi Huang,Baoming Tian,Gongyao Shi,Jinping Hua. 2022

[14]LEAF TIP RUMPLED 1 Regulates Leaf Morphology and Salt Tolerance in Rice. Wang, Jiajia,Liu, Yiting,Hu, Songping,Xu, Jing,Nian, Jinqiang,Cao, Xiaoping,Chen, Minmin,Cen, Jiangsu,Liu, Xiong,Zhang, Zhihai,Liu, Dan,Zhu, Li,Hu, Jiang,Ren, Deyong,Gao, Zhenyu,Shen, Lan,Dong, Guojun,Zhang, Qiang,Li, Qing,Yu, Sibin,Qian, Qian,Zhang, Guangheng. 2022

[15]Molecular Characterization and Gene Expression Analysis of Aquaporin in Haemaphysalis qinghaiensis. Qingli Niu,Rongzeng Hao,Yuping Pan,Zhijie Liu,Jifei Yang,Guiquan Guan,Jianxun Luo,Hong Yin. 2022

[16]Identification and Characterization of Salt-and Drought-Responsive AQP Family Genes in Medicago sativa L.. Yijing Luo,Lin Ma,Wenxuan Du,Su Yan,Zengyu Wang,Yongzhen Pang. 2022

[17]A composite vase solution improved vase quality of cut peony after long-term cold storage by maintaining better physiological activities and enhancing the absorption of water. Tai Chen,Caihuan Tian,Xiuxia Ren,Xiuxin Zhang,Jingqi Xue,Ruijie Hao. 2024

[18]Genome-wide analysis and expression of the aquaporin gene family in Avena sativa L.. Zhou, Xinyue,Yi, Dengxia,Ma, Lin,Wang, Xuemin. 2024

[19]Seeds Priming with Melatonin Improves Root Hydraulic Conductivity of Wheat Varieties under Drought, Salinity, and Combined Stress. Yuanyuan Fu,Penghui Li,Zhuanyun Si,Shoutian Ma,Yang Gao. 2024

[20]Cloning, expression, and functional identification of aquaporin genes from Eimeria tenella. Jing Wang,Zigang Qu,Xueting Ma,Joshua Seun Olajide,Jianping Cai. 2024

作者其他论文 更多>>