数字农科院2.0

A high-quality chromosome-level genome assembly of the oligophagous fruit fly Bactrocera tsuneonis (Diptera: Tephritidae) and insights into its host specificity

文献类型： 外文期刊

作者： Tengda Guo;Weisong Li;Yuan Zhang;Wenzhao Yang;Zhihong Li;Yujia Qin

作者机构：

关键词： Bactrocera tsuneonis;comparative genomics;genome;olfactory proteins;oligophagous

期刊名称： GigaScience

ISSN：

年卷期： 2025 年 14 卷

页码：

收录情况： SCIE（2025版）

摘要： BACKGROUND: Bactrocera tsuneonis is a major pest of citrus, causing significant economic losses in fruit production. It exhibits a highly specialized host preference, primarily infesting citrus fruits. However, the genetic basis underlying its olfactory adaptation and host specificity remains largely unexplored. To elucidate the molecular mechanisms governing host selection in B. tsuneonis, we assembled a high-quality chromosome-level genome and performed comparative genomic, transcriptomic, and functional analyses of its chemosensory system. RESULTS: The genome of B. tsuneonis was assembled to a total size of 339 Mb, with a contig N50 of 11.21 Mb and a scaffold N50 of 59.93 Mb. Comparative genomic analysis revealed significant contractions in chemosensory-related gene families, particularly in odorant-binding proteins (OBPs) and odorant receptors (ORs), perhaps suggesting an adaptation to a narrow host range. Transcriptome analysis demonstrated that BtsuOBP83a and BtsuOBP83b were highly expressed in the antennae, and most ORs were predominantly expressed in the antennae. Functional assays confirmed that BtsuOBP83a selectively binds to 2 citrus volatiles, trans-nerolidol and piperitone, with strong affinity. Molecular docking and molecular dynamics simulations further revealed that BtsuOR7a-6 and BtsuOR7a-4 specifically interact with these volatiles, suggesting their role in host odor recognition. CONCLUSIONS: Our high-quality genome of B. tsuneonis provides a valuable resource for genomic research and offers valuable insights into the genetic basis of its olfactory adaptation and host specificity. The findings highlight key molecular mechanisms underlying host selection and provide potential targets for behavior-based pest management strategies.

分类号：

  • 相关文献

[1]Genome analysis of phytophthora nicotianae jm01 provides insights into its pathogenicity mechanisms. Xiao Long Yuan,Cheng Sheng Zhang,Fan Yu Kong,Zhong Feng Zhang,Feng Long Wang. 2021

[2]Comparison of buckwheat genomes reveals the genetic basis of metabolomic divergence and ecotype differentiation. He, Ming,He, Yuqi,Zhang, Kaixuan,Lu, Xiang,Zhang, Xuemei,Gao, Bin,Fan, Yu,Zhao, Hui,Jha, Rintu,Huda, Md Nurul,Tang, Yu,Wang, Junzhen,Yang, Weifei,Yan, Mingli,Cheng, Jianping,Ruan, Jingjun,Dulloo, Ehsan,Zhang, Zongwen,Georgiev, Milen, I,Chapman, Mark A.,Zhou, Meiliang. 2022

[3]GRAND: An Integrated Genome, Transcriptome Resources, and Gene Network Database for Gossypium. Zhibin Zhang,Mao Chai,Zhaoen Yang,Zuoren Yang,Liqiang Fan. 2022

[4]四种甘蓝雄性不育类型基因芯片表达谱分析. 康俊根,王晓武,方智远. 2007

[5]Structural Basis and Recognition Mechanism of Host-Derived Volatiles by Olfactory Proteins in the Agricultural Pest Bactrocera correcta (Diptera: Tephritidae). Tengda Guo,Yuan Zhang,Yumeng Huang,Yongjun Zhang,Zhihong Li,Yujia Qin. 2025

[6]Entire Nucleotide Sequences Of Gossypium R.aimondii And G.Arboreum Mitochondrial G enomes Revealed A-Genome Species As Cytoplasmic Donor Of The Allotetraploid Species. Wang, M.,Pei, H.,Nie, H.,Zhao, Y.,Wendel, J. F.,Wang, Y.,Hua, J.,Grover, C. E.,Chen, Z.,Li, S.,Li, P.,Li, P.. 2017

[7]New cis-regulatory elements in the Rht-D1b locus region of wheat. Duan, Jialei,Wu, Jiajie,Liu, Yue,Xiao, Jianhui,Zhao, Guangyao,Jia, Jizeng,Kong, Xiuying,Gu, Yongqiang,Duan, Jialei. 2012

[8]Carotenoid biosynthetic genes in Brassica rapa: comparative genomic analysis, phylogenetic analysis, and expression profiling. Li, Peirong,Zhang, Shujiang,Zhang, Shifan,Li, Fei,Zhang, Hui,Cheng, Feng,Wu, Jian,Wang, Xiaowu,Sun, Rifei. 2015

[9]Comparative genomics provide a rapid detection of Fusarium oxysporum f. sp conglutinans. Ling Jian,Zhang Ji-xiang,Zeng Feng,Cao Yue-xia,Xie Bing-yan,Yang Yu-hong. 2016

[10]Diverse approaches to achieving grain yield in wheat. Zhang, Xueyong,Barrero, Roberto A.,Bellgard, Matthew.

[11]Artificial selection for determinate growth habit in soybean. Lee, Rian,McClean, Phillip E.,Lee, Rian,McClean, Phillip E.,Wang, Xiaobo,Li, Yinghui,Qiu, Lijuan,Tian, Zhixi,Ma, Jianxin,Specht, James E.,Nelson, Randall L.,Nelson, Randall L..

[12]Structural organization of the barley D-hordein locus in comparison with its orthologous regions of wheat genomes. Gu, YQ,Anderson, OD,Londeore, CF,Kong, XY,Chibbar, RN,Lazo, GR. 2003

[13]Application of comparative genomics in developing markers tightly linked to the Pm-2F gene for powdery mildew resistance in melon (Cucumis melo L.). Zhang, Chunqiu,Ren, Yi,Guo, Shaogui,Zhang, Haiying,Gong, Guoyi,Xu, Yong,Zhang, Chunqiu,Du, Yongchen.

[14]Sinbase: An Integrated Database to Study Genomics, Genetics and Comparative Genomics in Sesamum indicum. Wang, Linhai,Yu, Jingyin,Li, Donghua,Zhang, Xiurong.

[15]Identification of cotton microRNAs and their targets. Baohong Zhang,Qinglian Wang,Kunbo Wang,Xiaoping Pan,Fang Liu,Tenglong Guo,George P. Cobb,Todd A. Anderson. 2007

[16]Escape from preferential retention following repeated whole genome duplications in plants. Schnable, James C.,Freeling, Michael,Wang, Xiaowu,Pires, J. Chris. 2012

[17]The complete mitochondrial genome of the tapeworm Cladotaenia vulturi (Cestoda: Paruterinidae): gene arrangement and phylogenetic relationships with other cestodes. Guo, Aijiang,Guo, Aijiang. 2016

[18]Anthocyanin biosynthetic genes in Brassica rapa. Guo, Ning,Cheng, Feng,Wu, Jian,Liu, Bo,Zheng, Shuning,Liang, Jianli,Wang, Xiaowu. 2014

[19]Comparative Analysis of Asteraceae Chloroplast Genomes: Structural Organization, RNA Editing and Evolution. Cui, Licao,Feng, Kewei,Deng, Pingchuan,Du, Xianghong,Song Weining,Nie, Xiaojun,Wang, Mengxing,Cui, Licao,Feng, Kewei,Deng, Pingchuan,Du, Xianghong,Song Weining,Nie, Xiaojun,Wan, Fanghao.

[20]Glucosinolate biosynthetic genes in Brassica rapa. Wang, Xiaowu. 2011

作者其他论文 更多>>