数字农科院2.0

Rapid Detection of Prometryn Residues in Agricultural Samples: Method Comparison of the ic-ELISA and Colloidal Gold Immunochromatographic Test Strips

文献类型： 外文期刊

作者： Gao, Song;Abd El-Aty, A. M.;Xu, Lingyuan;Zhao, Jing;Li, Jia;Lei, Xingmei;Zhao, Yun;She, Yongxin;Jin, Fen;Wang, Jing;Yuan, Sichun;Jin, Maojun;Zheng, Lufei;Hammock, Bruce D.

作者机构：

关键词： prometryn;ic-ELISA;colloidal gold immunochromatographictest strip;agricultural products

期刊名称： ACS AGRICULTURAL SCIENCE & TECHNOLOGY

ISSN：

年卷期： 2024 年

页码：

收录情况： 无来源刊（2024版）

摘要： This study aimed to establish two methods, namely, indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and colloidal gold immunochromatographic test strips, for detecting prometryn residues in various agricultural samples, such as corn, pumpkin, lotus root, rice, and brown rice. Monoclonal antibodies specific for prometryn were utilized in both methods. The ic-ELISA method demonstrated a maximum half-inhibition concentration (IC50) of 2.4 ng/mL and a lower limit of detection of 0.5 ng/mL. When used in conjunction with a card reader, the colloidal gold immunochromatographic test strip exhibited an IC50 of 18.6 ng/mL for prometryn detection. The recovery rates of both methods were found to be consistent with those of liquid chromatography tandem mass spectrometry. This consistency indicated the suitability of both methods for detecting prometryn in five different agricultural samples, suggesting that this is a convenient and practical approach for screening prometryn in agricultural products.

分类号：

  • 相关文献

[1]Characterization and catalytic mechanism of a direct demethylsulfide hydrolase for catabolism of the methylthiol-s-triazine prometryn. Tang J.,Yao J.,Pan D.,Huang J.,Wang J.,Li Q.X.,Dong F.,Wu X.. 2023

[2]Obtaining a Monoclonal Antibody against a Novel Prometryn-Like Hapten and Characterization of Its Selectivity for Triazine Herbicides. Lingyuan Xu,A. M. Abd El-Aty,Jing Zhao,Xingmei Lei,Xiuyuan Zhang,Yun Zhao,Xueyan Cui,Yongxin She,Fen Jin,Jing Wang,Maojun Jin,Bruce D. Hammock. 2023

[3]Development of a Highly Sensitive Time-Resolved Fluorescence Immunochromatographic Assay for Rapid Onsite Determination of Prometryn Residues in Food Samples. Zhao, Jing,Gao, Song,Li, Peipei,Abd El-Aty, A. M.,Xu, Lingyuan,Jin, Fen,Wang, Jing,Li, Jia,Lin, Weiping,Zheng, Lufei,Jiao, Bining. 2026

[4]Production of a Monoclonal Antibody for the Detection of Forchlorfenuron: Application in an Indirect Enzyme-Linked Immunosorbent Assay and Immunochromatographic Strip. Xingmei Lei,A. M. Abd El-Aty,Lingyuan Xu,Jing Zhao,Jia Li,Song Gao,Yun Zhao,Yongxin She,Fen Jin,Jing Wang,Lufei Zheng,Maojun Jin,Bruce D. Hammock. 2023

[5]Preparation of specific nanobodies and their application in the rapid detection of nodularin-r in water samples. Jinyi Yang,Rui Si,Guangpei Wu,Yu Wang,Ruyu Fang,Fei Liu,Feng Wang,Hongtao Lei,Yudong Shen,Qi Zhang,Hong Wang. 2021

[6]Generation of a highly specific recombinant full-length antibody for detecting ethirimol in fruit and environmental water. Xingmei Lei,Peipei Li,A. M. Abd El-Aty,Jing Zhao,Lingyuan Xu,Song Gao,Jia Li,Yun Zhao,Yongxin She,Fen Jin,Jing Wang,Lufei Zheng,Bruce D. Hammock,Maojun Jin. 2024

[7]Development of a Monoclonal Antibody-Based Indirect Competitive Enzyme-Linked Immunosorbent Assay for the Rapid Detection of Gallic Acid. Jiajing Duan,Xiuxia Zheng,Ran Tao,Long Li,Fengzhong Wang,Yufeng Sun,Bei Fan. 2024

[8]Preparation of monoclonal antibodies recognizing pharmacologically active metabolites of metamizole based on rational hapten design and their application in the detection of animal-derived food. Linwei Zhang,Jiacan Wang,Yiting Wang,Hao Wen,Mingyue Ding,Jiaxu Xiao,Hongfei Yang,Xiaoming Pan,Shiyun Han,Dapeng Peng. 2024

[9]Using Quantile Regression Approach to Analyze Price Movements of Agricultural Products in China. Li Gan-qiong,Xu Shi-wei,Li Zhe-min,Dong Xiao-xia,Sun Yi-guo. 2012

[10]Considerations Of Constructing Quality, Health A.nd Safety Management System F or Agricultural Products Sold Via E-Commerce. Ma, C, Wang, DY, Hu, ZQ, Li, Z. 2018

[11]Application and Preparation Progress of Stable Isotope Reference Materials in Traceability of Agricultural Products. Shanshan Zhao,Yan Zhao. 2021

[12]Insights into stress degradation behavior of gibberellic acid by UHPLC Q-Exactive Orbitrap mass spectrometry. Jiang C., Han H., Dai J., Wang Z., Chai Y., Wang C., Liu X., Lu C., Chen H.. 2022

[13]Preparation of magnetic molecularly imprinted polymers for the selective extraction of strobilurin fungicides in agricultural products. Yexuan Hu,Tongqing Liu,Shuanglei Wang,Meijin Lv,Yongxin She,Bin Cao,Ziping Zhang,Xiaolin Cao. 2023

[14]Recent Advances in Rapid Detection Techniques for Pesticide Residue: A Review. Xu L.,Abd El-Aty A.M.,Eun J.-B.,Shim J.-H.,Zhao J.,Lei X.,Gao S.,She Y.,Jin F.,Wang J.,Jin M.,Hammock B.D.. 2022

[15]Quantifying supply chain food loss in China with primary data: A large-scale, field-survey based analysis for staple food, vegetables, and fruits. Shijun Lu,Guangyan Cheng,Tai Li,Li Xue,Xiaojie Liu,Jiazhang Huang,Gang Liu. 2022

[16]Preparation of metal–organic framework @molecularly imprinted polymers for extracting strobilurin fungicides from agricultural products. Liu T.,Hu Y.,Feng Y.,Liu Y.,Xie P.,Chai L.,Qi Y.,Cong S.,She Y.,Cao X.. 2022

[17]Synthesis of metal-organic framework @molecularly imprinted polymer adsorbents for solid phase extraction of organophosphorus pesticides from agricultural products. Yang Li,Bingzhi Li,Yan Qi,Ziping Zhang,Shuang Cong,Yongxin She,Xiaolin Cao. 2022

[18]Detection of neonicotinoids in agricultural products using magnetic molecularly imprinted polymers-surface enhanced Raman spectroscopy. Cao, Xiaolin,Hu, Yexuan,Yu, Huimin,Sun, Shuai,Xu, Dan,Zhang, Ziping,Cong, Shuang,She, Yongxin. 2024

[19]Effect of different agricultural conditions, practices, and processing on levels of total arsenic and species in cereals and vegetables: A review. Yanfang Liu,Yatao Huang,Lin Li,Yangyang Xiong,Litao Tong,Fengzhong Wang,Bei Fan,Jiashun Gong. 2023

[20]Smartphone-integrated visual inspection for enhancing agricultural product quality and safety: a review. Xu, Lingyuan,Abd El-Aty, A. M.,Li, Peipei,Li, Jia,Zhao, Jing,Lei, Xingmei,Gao, Song,Zhao, Yun,She, Yongxin,Jin, Fen,Wang, Jing,Wang, Shuting,Zheng, Lufei,Hammock, Bruce D.,Jin, Maojun. 2024

作者其他论文 更多>>